浒苔多糖对羟自由基的清除作用研究  

Study on the scavenging activity of polysaccharides from Ente-romorpha prolifera to hydroxyl radical

作　　者：薛丁萍[1] 魏玉西[1] 刘淇[2] 刘蕾[1] 

机构地区：[1]青岛大学生物系,山东青岛266071 [2]中国水产科学研究院黄海水产研究所,山东青岛266071

出　　处：《海洋科学》2010年第1期44-47,共4页Marine Sciences

基　　金：国家科技支撑计划项目(2008BAC49B04);青岛市科技计划项目(08-1-7-6-hy)

摘　　要：研究确定了Fenten(水杨酸法)反应所需水杨酸、H2O2和Fe2+的浓度,并用水杨酸法对浒苔(Entero-morpha prolifera)水提多糖及其酸提多糖对羟自由基的清除能力进行了测定,同时,与合成抗氧化剂BHT和TBHQ进行对比。结果表明,浒苔水提多糖及酸提多糖对羟自由基均有一定的清除效果,且清除率与浓度存在剂量依赖关系,酸提多糖对羟自由基的清除能力大于水提多糖。在较高质量浓度(>1.2 g/L)时酸提多糖对羟自由基的清除能力是水提多糖的2倍。The concentrations of salicylic acid, H2O2 and Fe2＋ needed in Fenten reaction model were examined firstly in this paper. Polysaccharides extracted by water （PW） and acid （PA） from Enteromorpha were prepared, and then their anti-oxidative activities were evaluated by radicals scavenging ratio to · OH and compared with synthesized antioxidants BHT and TBHQ. The results showed that PW and PA all had certain activities to scavenge · OH and in a dose-dependent manner, and PA presented higher scavenging ratio to · OH than PW. At the sample concentration of more than 1.2 g/L for PA, the scavenging ratio to · OH doubled compared with PW. Therefore, it was suggested that polysaceharide by acid extraction from Enteromorpha should be a strong · OH scavenger and thus has a bright future in food industry.

关 键 词：浒苔( ENTEROMORPHA PROLIFERA ) 多糖 羟自由基 清除率 

分 类 号：R151[医药卫生—营养与食品卫生学]