竹叶功能因子生物抗氧化性质的研究  

THE BIO ANTIOXIDATIVE ACTIVITY OF FUNCTIONAL FACTORS IN BAMBOO LEAVES

作　　者：张英[1,2] 唐莉莉[1,2] 丁霄霖[1,2] 

机构地区：[1]浙江农业大学食品科技系 [2]无锡轻工大学食品学院

出　　处：《营养学报》1998年第3期367-371,共5页Acta Nutrimenta Sinica

基　　金：国家"八五"攻关课题

摘　　要：目的：研究竹叶功能因子的生物抗氧化性质。方法：实验一，在用化学发光法（ＣＬ）考察发现竹叶提取物（ＢａｍｂｏｏＬｅａｆＥｘｔｒａｃｔｓ，ＢＬＥ）普遍具有较强的抗活性氧自由基效能的基础上，测定竹叶功能因子（ＢａｍｂｏｏＬｅａｆＦｕｎｃｔｉｏｎａｌＦａｃｔｏｒｓ，ＢＬＦＦ）清除Ｏ２和ＯＨ的有效浓度范围。结果：分别在０．１～１０ｍｇ／Ｌ和１０μｇ～１ｇ／Ｌ之间。在Ｘ－ＸＯＤ－Ｌｕｍｉｎｏｌ的Ｏ２产生体系中，当ＢＬＦＦ试样浓度加大到５ｇ／Ｌ（相当于６３００倍的ＩＣ５０）时，抑制率仍稳定在１００％左右；在ＶＣ－Ｃｕ２＋－Ｈ２Ｏ２酵母多糖的ＯＨ产生体系中，当试样浓度达到５ｇ／Ｌ时，同样没有表现体外促氧化作用，表明ＢＬＦＦ有着优良和稳定的抗氧化活性。实验二，用邻苯三酚（ＰＲ）自氧化法和电子自旋共振法（ＥＳＲ）测定了不同竹叶制剂对Ｏ２的清除作用，从多个体系和不同手段进行验证。结果：与ＣＬ具有显著的相关性。实验三，观察竹叶有效成分对生物体内源性抗氧化酶系的诱导作用。以蜂花粉为阳性对照，进行老龄小鼠的ＢＬＥ喂养试验，连续灌胃３５天后，用ＣＬ和ＤＴＮＢ法测定全血的活力。结果：ＢＬＥ能显著提高衰老小鼠体内ＳＯＤ和ＧＳＨ－Ｐｘ的活力。?Bamboo leaves contain abundant flavonoids and other bio active substances which have excellent scavenging capacity on reactive oxygen radicals.Methods and Results: (a) The prior works by chemiluminesence (CL) showed the bamboo leaf extracts (BLE) possessed generally strong scavenging capacity on reactive oxygen radicals. The effective concentrations of bamboo leaf functional factors (BLFF) were in the rangs of 0.1-10mg/L on O · 2 and 10μg-1g/L on ·OH respectively. In X XOD Luminol model which produces O · 2, BLFF at 5g/L, equal to 6 300 times of IC 50 , still scavenged O · 2 entirely; and in the VC Cu 2+ H 2O 2 yeast polysaccharide system which produces ·OH, BLFF at 5g/L did not contribute prooxidant effect too. These revealed that the effective fractions in bamboo leaves have excellent and stable antioxidative activity. (b) Multimedia experiments, PR auto oxidation and ESR were also used to evaluate the scavenging capacity on O · 2 of several preparations of bamboo leaves. Results coincided with that of CL. (c) In order to investigate the effect of BLE on endo antioxidases in vivo, a feeding test of aged mice was done taking bee pollen as control. After 35 days,the results showed that BLE significantly promoted the activities of SOD and GSH Px of aged mice. In combination with the experimental results both in vitro and in vivo, it is attained that bamboo leaves contain a class of functional factor which has the effect of anti free radicals and anti oxidation.

关 键 词：竹叶制剂 功能因子 氧自由基清除剂 抗氧化作用 

分 类 号：R977.9[医药卫生—药品] R151.3[医药卫生—药学]