BRCA1有关微卫星多态与乳腺癌生物学特性的关系  

THE ASSOCIATION BETWEEN THE POLYMORPHISM OF BRCA1 RELATED MICROSATELLITES AND THE BIOLOGICAL BEHAVIORS OF SPORADIC BREAST CANCER

作　　者：沈坤炜[1] 袁建达[1] 张亚伟[1] 崔静[2] 沈福民[2] 韩企夏[1] 沈镇宙[1] 

机构地区：[1]上海医科大学附属肿瘤医院,上海200032 [2]上海医科大学流行病教研室,上海200032

出　　处：《中国癌症杂志》1998年第3期157-159,共3页China Oncology

摘　　要：目的了解BRCA1附近的4个多态标记位点在散发性乳腺癌中发生LOH（杂合性丢失）和MSI（微卫星不稳定性）的情况，探讨LOH和MSI和乳腺癌临床病理参数之间的关系。方法应用聚合酶链式反应（PCR）一变性聚丙烯酰胺凝胶电泳（PAUGE）－DNA银染方法。结果4个多态位点总的LOH和MSI的发生率分别为62．5％和45．0％。肿瘤最大径≤2cm的患者MSI的发生率显著高于肿瘤最大径＞2cm的患者（P<O．05），I期乳腺癌MSI的发生率高于Ⅱ期以上的乳腺癌，差异接近显著（P=0．082）。LOH的情况和MSI相反，但差异未达显著。MSI和LOH的发生率和患者月经状况、淋巴结有否转移及雌激素受体状况无关。结论4个多态位点附近存在乳腺癌易感基因。MSI可能是乳腺肿瘤发生早期阶段的遗传物质的改变，而LOH则可能是乳腺肿瘤发生晚期阶段的遗传物质的改变。PURPOSE To study the frequency of LOH (loss of heterozygosity) and MSI (microsatellite instability)of fourBRCAl related polymorphic markers in sporadic breast cancer and to correlate their incidence with clinicopathological parameters. METHODS Polymerase chain reaction-polyacrylamide urea gel electrophoresis-DNA silverstaining was used. RESULTS The total incidence of LOH and MSI of four BRCAl related polymorphic markers inbreast cancer were 62. 5 % and 45. 0%,respectively. The incidence of MSI in patients with tumours 2 cm in diameter or less was higher than those with tumours more than 2 cm in diameter (P<0. 05). There is a tendency for theincidence of MSI to be higher in stage I breast cancer patients than later stage cases,while the contrary exists forLOH. The incidence of LOH and MSI was not correlated to menstrual,lymphnode and estrogen receptor status.CONCLUSION Breast cancer susceptibility gene was located near these four microsatellites DNA. MSI may be anearly event in mammary tumorigenesis, while LOH may occur at later stage.

关 键 词：乳腺肿瘤 多态标记 BRCA1 微卫星不稳定性 

分 类 号：R737.902[医药卫生—肿瘤]