人乳腺癌细胞株增殖细胞核抗原表达及溴脱氧尿核苷参入的研究  

CORRELATION OF EXPRESSION OF PROLIFERATING CELL NUCLEAR ANTIGEN (PCNA) AND BROMODEOXYURIDINE LABELLING IN HUMAN BREAST CANCER CELL LINE MCF-7

作　　者：张亚伟[1] 江明[1] 邵志敏[1] 施达仁[2] 沈镇宙[1] 

机构地区：[1]上海医科大学附属肿瘤医院外科,上海200032 [2]上海医科大学附属肿瘤医院病理科,上海200032

出　　处：《中国癌症杂志》1998年第3期169-171,共3页China Oncology

摘　　要：目的研究人乳腺癌细胞株（MCF－7）中增殖细胞核抗原（PCNA）的表达与细胞增殖的关系。方法对人乳腺癌细胞株MCF－7传代后培养，每天收获部分细胞，连续8天，分别作增殖细胞核抗原（PCNA）检测及溴脱氧尿核苷（BrdU）参入测定，比较PCNA阳性细胞标记指数（PCNALI）与BrdU标记指数（BrdULI）的动态变化的相关性。结果在MCF－7细胞传代培养中，PCNALI与BrdULI两个指标之间存在着显著的相关性（γ=0．92，P＜0．01）。结论PCNALI作为细胞增殖指标，为细胞增殖动力学变化提供了可靠信息。PURPOSE To study the role of proliferating cell nuclear antigen (PCNA) expression in MCF-7 cells aftersubculture. METHODS Proliferating cell nuclear antigen (PCNA) expression was assessed compared with proliferation-associated marker bromodeoxyuridine (BrdU) in human breast cancer cell MCF-7. The daily PCNA labelling index (PCNALI) and BrdU labelling index (BrdULI) had been determined for 8 days after subculture ofMCF-7 cells. RESULTS The correlation between daily PCNALI and BrdULI was very strong (γ=0. 92, P<0.01). CONCLUSION PCNALI can be used as a reliable marker to indicate cell proliferative activity in MCF-7cells.

关 键 词：乳腺癌 细胞株 增殖细胞核抗原 溴脱氧尿核苷 

分 类 号：R737.902[医药卫生—肿瘤]