直肠癌DNA含量的检测及临床意义  

Detection of DNA content in the distal bowel of rectal carcinoma and its clinical significance

作　　者：刘志勇[1] 李晓林[2] 王彦升[2] 宋茂力[2] 孙培胜[2] 邹小明[2] 

机构地区：[1]赣南医学院第一附属医院乳腺外科,江西赣州341100 [2]哈尔滨医科大学第二临床医学院胃肠外科,黑龙江哈尔滨150081

出　　处：《哈尔滨医科大学学报》2009年第6期568-571,共4页Journal of Harbin Medical University

摘　　要：目的检测直肠癌及癌旁组织中DNA含量以探讨直肠癌远端肠管的切除范围。方法采用流式细胞分析技术对60例直肠癌标本的肿瘤组织(T)、远端1cm(P1)、远端2cm(P2)、远端3cm(P3)、远端4cm(P4)、远端5cm(P5)及正常组织(N)进行检测,分析各部位组织中DNA指数(DI)的差异性。结果T组织异倍体率显著高于P1～P5组织及N组织,P4组织异倍体率显著高于P5及N组织,而P5与N组织差异无显著性。结论直肠癌远端1～4cm肠管中存在着DNA含量的异常改变,呈现出恶性肿瘤生物学特性。手术时应切除癌旁超过4cm作为安全切除范围。Objective To explore the optimal distence of distal resection margin of rectal carcinoma by detecting DNA contents. Methods Sixty cases of colorectal carcinoma （T）,tissue 1 cm （P1）,2 cm （P2）,3 cm（P3）,4 cm（P4）,5 cm（P5） away from tumor margin and the normal tissue （N） were detected by the flow cytometry. DNA index （DI） were analyzed. Results The aneuploidy rate in tumors were significantly higher than that in P1 ～ P5 and normal tissues,meanwhile the rate in P4 significantly higher than that in P5 and normal tissues. Conclusion The abnormal change of DNA contents can be detected in the 4 cm distal rectum, it shows the tendency of malignancy. Beyond 4 cm tissue of tumor margin should be removed in curative procedure for rectal carcinoma.

关 键 词：直肠肿瘤 流式细胞技术 DNA指数 

分 类 号：R735.37[医药卫生—肿瘤]