血清总胆汁酸双试剂连续监测的临床意义  

Clinical significance of consecutive monitoring of serum total bile acids with double reagent

作　　者：葛鑫[1,2] 刘坤[1,2] 李兴武 杜军[1,2] 

机构地区：[1]蚌埠医学院附属医院检验中心 [2]蚌埠市第三人民医院检验科

出　　处：《蚌埠医学院学报》1998年第5期358-359,共2页Journal of Bengbu Medical College

摘　　要：目的：利用全自动生化分析仪，建立一种快速检测血清总胆汁酸（ＴＢＡ）活性的方法和正常参考值。方法：双试剂酶偶联连续监测法，检测６０例不同肝病患者和３００名健康成人血清ＴＢＡ活性。结果：其测值（ｘ±ｓ）分别为４８．９５±５．７３μｍｏｌ／Ｌ和５．１２±３．４６μｍｏｌ／Ｌ，差异有显著性（Ｐ＜０．０１）；本法批内ＣＶ分别为１．９９％、２．８５％和３．００％，批间ＣＶ分别为３．２８％、３．４０％和３．１６％，线性范围０～２００μｍｏｌ／Ｌ。结论：双试剂酶偶联连续监测法，线性好，特异性强，灵敏度高，快速、准确，对肝脏疾病的诊断及临床分级具有重要价值。Objective:To establish a quick and ideal method for detection of serum total bile acids(TBA) and its normal reference range with autobiochemical analysis instrument.Methods:Serum TBA in 60 cases of different hepatopathies and 300 cases of healthy adults were detected consecutively with double reagents by enzyme linking.Results:Serum TBA of different hepatopathies and normal adults were 48.95±5.73 μmol/L and 5.12±3.46 μmol/L respectively.This difference was significant( P <0.01).Intra assay CV of the method were 1.99 %, 2.85 % and 3.0% respectively,Inter assay CV were 3.28%,3.4% and 3.16% respectively,the range of linearity from 0 to 200 μmol/L.Conclusions:The results show that this method had a good linearity range,high specificity and sensitivity,and is quick and accurate.The determination of serum TBA has important clinical significance for the diagnosis and grading of different hepatopathies.

关 键 词：总胆汁 肝疾病 酶复剂 血清诊断 

分 类 号：R575.04[医药卫生—消化系统] R446.112[医药卫生—内科学]