1α,25-二羟维生素D_3对前列腺癌LNCaP细胞中E-cadherin调节作用的研究  

Regulation of E-cadherin in prostate cancer cell line LNCaP

作　　者：李纲[1] 郭震华[1] 影山幸雄 大岛博幸 

机构地区：[1]苏州医学院附属第一医院泌尿外科,215006 [2]东京医科齿科大学医学部附属病院泌尿科

出　　处：《临床泌尿外科杂志》1998年第10期451-454,共4页Journal of Clinical Urology

摘　　要：为探讨1α，25－二羟维生素D_3与前列腺癌转移之间的关系，采用WesternBlot印迹及免疫荧光法分析了前列腺癌细胞林LNCaP细胞中E－cadherin的表达，用逆转录聚合酶链反应分析了E－cadherinmRNA的表达。结果当培养液中加入0．01、0．1和1．0nmol／L浓度的1α，25-二羟维生素D3时，LNCaP细胞中E－cadherin表达增强，当浓度增加到10和100nmol／L时，E－cadherin表达减少。E－cadherinmRNA变化与E－cadherin表达变化相一致。结果显示1α，25－二羟维生素D3在低浓度时能促进转移抑制物质E－cadherin的表达，高浓度时直接抑制癌细胞的增殖。认为1α，25－二羟维生素D3可能抑制前列腺癌细胞株LNCaP细胞的转移能力。The effect of 1α, 25-dihydroxyvitamin D3 on the E-cadherin expression in a human prostatic carcinoma cell line LNCaP was investigated to discuss a possible Inechanism of 1α, 25-dihydroxyvitamin D3 to prevent progression of prostatic carcinoma. E-cadherin expression in LNCaP cells was evaluated by Western blot and immunofluorescent analysis. The E-cadherin mRNA level was estimated by reverse-transcriptase polymerase chain reaction. The E-cadherin expression was enhanced by the presence of 0. 01 .0. 1 and 1. 0 nmol/L 1α, 25-dihydroxyvitamin D3 in culture medium and then reduced by further increase of 1α, 25-dihydroxyvitamin D3 concentrations to 10 and 100nmol/L- The E-cadherin mRNA levels changed in accordance with the above protein expression.1α, 25-dihydroxyvitamin D3 inhibited the proliferation of LNCaP cells at 10 and 100 nmol/L concentrations. The results indicate that 1α, 25-dihydroxyvitamin D3 enhances the expression of E-cadherin in LNCaP cells at low concentrations,while it excerts direct antiproliferative ability and reduces Ecadherin expression at high concentrations.

关 键 词：前列腺肿瘤 细胞粘附分子 维生素D LNCAP细胞 

分 类 号：R737.250.2[医药卫生—肿瘤]