Comparative study of microbial community structure in different filter media of constructed wetland  

作　　者：Ming Li Qiaohong Zhou Min Tao Ying Wang Lijuan Jiang Zhenbin Wu 

机构地区：[1]State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China [2]Graduate University of Chinese Academy of Sciences, Beijing 100039, China [3]School of Resource & Environmental Engineering, Wuhan University of Technology, Wuhan 430070, China

出　　处：《Journal of Environmental Sciences》2010年第1期127-133,共7页环境科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (No.30870221,20877093,50808172);the High Technology Research and Development Program (863) of China (No.2009X207209-004)

摘　　要：Comparisons of microbial community structure, in eight filter media of zeolites, anthracite, shale, vermiculite, ceramic filter media, gravel, steel slag and bio-ceramic, were undertaken by analyzing the phospholipid fatty acid （PLFA） composition. A total of 20 fatty acids in the range of C18to C20 were determined but only 13 PLFAs were detected in steel slag. They consist of saturated fatty acids, branched fatty acids, monounsaturated fatty acids, and polyunsaturated fatty acids. The variation of fatty acids was revealed in the relative proportions of these fatty acids in different media. The aerobic prokaryotes were the predominant group in all media. The PLFA composition showed significant differences among the eight different media by Tukey＇s honestly test. It was found that steel slag was significantly different in the microbial community as compared to other filter media, probably due to its alkaline effluent. Steel slag alone is probably not a good choice of substratum in constructed wetlands. The principle components analysis （PCA） showed that zeolites, bio-ceramic, shale and vermiculite had a similar microbial community structure while steel slag and ceramic filter media were distinct from other media.Comparisons of microbial community structure, in eight filter media of zeolites, anthracite, shale, vermiculite, ceramic filter media, gravel, steel slag and bio-ceramic, were undertaken by analyzing the phospholipid fatty acid （PLFA） composition. A total of 20 fatty acids in the range of C18to C20 were determined but only 13 PLFAs were detected in steel slag. They consist of saturated fatty acids, branched fatty acids, monounsaturated fatty acids, and polyunsaturated fatty acids. The variation of fatty acids was revealed in the relative proportions of these fatty acids in different media. The aerobic prokaryotes were the predominant group in all media. The PLFA composition showed significant differences among the eight different media by Tukey＇s honestly test. It was found that steel slag was significantly different in the microbial community as compared to other filter media, probably due to its alkaline effluent. Steel slag alone is probably not a good choice of substratum in constructed wetlands. The principle components analysis （PCA） showed that zeolites, bio-ceramic, shale and vermiculite had a similar microbial community structure while steel slag and ceramic filter media were distinct from other media.

关 键 词：microbial community structure PHOSPHOLIPID filter media biomarkers constructed wetland steel slag 

分 类 号：X703.1[环境科学与工程—环境工程] TQ546.5[化学工程—煤化学工程]