Ⅱ组代谢型谷氨酸受体阻断剂对大鼠海马神经元凋亡的影响  

Effects of group-Ⅱ metabotropic glutamate receptor antagonist on apoptosis of neurons in rat hippocampus

作　　者：冯荣芳[1] 刘凌云[2] 王建华[1] 吕佩源[1] 石卫东[3] 陈景红[1] 冯亚青[1] 王天俊[1] 

机构地区：[1]河北省人民医院神经内科,石家庄050051 [2]上海市杨浦区中心医院神经科 [3]河北省人民医院病理科

出　　处：《中华老年心脑血管病杂志》2010年第1期73-76,共4页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

摘　　要：目的观察Ⅱ组代谢型谷氨酸受体阻断剂对大鼠海马神经元凋亡的影响。方法 SD大鼠24只随机分为假手术组、痴呆组和阻断剂组,每组8只。大鼠脑室注射凝聚肽Aβ_(25-35)5建立痴呆大鼠模型,阻断剂组1周后行阻断剂脑室注射,另2组等量注射人工脑脊液4周行Morris水迷宫测试;测试1周后取材行病理观察,采用TUNEL法检测海马CA1区锥体细胞凋亡情况。结果与假手术组比较,痴呆组大鼠平均潜伏期明显延长,平台象限滞留时间明显缩短(P<0.05);与痴呆组比较,阻断剂组大鼠上述指标明显提高(P<0.05)。与假手术组比较,痴呆组大鼠海马CA1区可见大量的凋亡锥体细胞,阳性锥体细胞数、总面积、平均吸光度(A)值明显升高(P<0.05);与痴呆组比较,阻断剂组大鼠海马CA1区可见明显的阳性细胞和核固缩,但其阳性染色锥体细胞数、总面积、平均A值明显降低(P<0.05)。结论Ⅱ组代谢型谷氨酸受体阻断剂可明显抑制痴呆引起的海马CA1区锥体细胞的凋亡,提示其可能通过影响细胞凋亡,参与痴呆的发病过程。Objective To observe the effects of the antagonist of metabotropic glutamate receptor 2/3（mGluR2/3） on apoptosis of hippocampal neurons. Methods Twenty-four Sprague Dawley rats were randomly divided into 3 groups：sham-operated, Alzheimer＇s disease（AD）, and a-methyl-（4-tetrazolyl-phenyD glycine（MTPG） groups. The dementia rat model was established by injecting agglomerated Aβ25-35 into the lateral ventricle, then MTPG was injected into ventricle 1 week later in MTPG group. Morris water maze test was performed 4 weeks after CSF or MTPG injection. Apoptosis of pyramidal neurons in the CA1 subfield of hippocampus was examined using TUNEL labeling method 1 week after Morris water maze test. Results The ability in learning and memorizing in the dementia group significantly decreased as compared with that in the shamoperated group （P 〈 0.05） ;the ability in learning and memorizing in MTPG group was much improved as compared with that in the dementia group. There were numerous apoptotic pyramidal cells in CA1 subfield of hippocampus,the number,total area and average light density of the positive pyramidal cells were significantly increased in the AD group compared with those in sham-operated group （P 〈 0.05）. In the MTPG group,the number,total area and average light density of the positively stained pyramidal cells were significantly decreased compared with those in the AD group （P〈0.05）. Conclusion MTPG could significantly inhibit the apoptosis of pyramidal neurons in the CA1 subfield of hippocampus caused by AD,suggesting that mGluR2/3 might participate in the process of AD by influencing apoptosis.

关 键 词：受体 谷氨酸 海马 神经元 细胞凋亡 痴呆 

分 类 号：R363[医药卫生—病理学]