溴氰菊酯降解菌Pseudomonas sp.P1-1-B3产酶条件的优化  

Optimal Conditions on the Production of Deltamethrin-degrading Enzyme from Pseudomonas sp.P1-1-B3 Strain

作　　者：刘丽花[1] 王兆守[1] 陈小兰[1] 徐敦明[2] 郭英霞[1,3] 邵宗泽[4] 

机构地区：[1]厦门大学化学化工学院,福建厦门361005 [2]厦门出入境检验检疫局,福建厦门361026 [3]厦门大学环境科学研究中心,福建厦门361005 [4]国家海洋局第三海洋研究所海洋生物遗传资源重点实验室,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2010年第1期71-76,共6页Journal of Xiamen University：Natural Science

基　　金：福建省青年科技人才创新项目(2007F3094);厦门大学引进人才科研启动费项目(0000-X071C3);细胞生物学与肿瘤细胞工程教育部重点实验室(厦门大学)开放基金(2007110);近海海洋环境科学国家重点实验室(厦门大学)开放基金(MEL0603);国家海洋局第三海洋研究所海洋生物遗传资源重点实验室开放研究基金(HY200601);国家海洋局近岸海域生态环境重点实验室开放基金(200702)

摘　　要：农药降解酶对去除农药残留污染具有良好的应用前景.从海洋沉积物中筛选到一株高产溴氰菊酯降解酶的菌株Pseudomonas sp.P1-1-B3,研究了碳源、氮源、pH值、培养温度、培养时间、接种量及溴氰菊酯对菌株产酶的影响.结果表明,降解菌产酶的最适条件为:以可溶性淀粉作为碳源,m(蛋白胨):m(酵母浸膏)=2:1为氮源,pH 7.5,温度30℃,培养时间2 d,接种量3%,溴氰菊酯含量15μmol/L.在此条件下,菌株产酶的比活力最大为113.3 U/mg.该降解酶对溴氰菊酯的降解,在12 h内降解率达54.6%.The pesticide-degrading enzyme shows good prospective application for the removal of pesticide residues. Some optimal conditions were studied on producing deltamethrin-degrading enzyme from Pseudornonas sp. P1-1-B3 strain, which obtained from marine sediment and showed high degradation efficiency on deltamethrin. These conditions consisted of carbon sources, nitrogen sources, pH value, temperature, cultivation time,inoculation amount of bacteria and concentration of deltamethrin. The results showed that the maxium enzymatic activity 113.3 U/mg when the strain was cultured under the following conditions：soluble starch as carbon source, the mass ratio of peptone to yeast extract （2 ： 1）, pH 7. 5,30 ℃, 3 % inoculation amount of bacteria, 2 d of cultivation time, 15 μmol/L of deltamethrin in media. Meanwhile, the degrading enzyme showed good degradation efficiency on deltamethrin with the degradation rate of 54.6% in 12 h.

关 键 词：溴氰菊酯 菌株Pseudomonas sp.P1—1-B3 降解酶 产酶条件 优化 

分 类 号：Q935[生物学—微生物学]