黑色型黄粉虫不同发育时期酯酶和过氧化物酶同工酶的比较研究  

Comparison on Esterase Isozyme and Peroxidase Isozyme of Black Worm Tenebrio molitor Linnaeus at Different Developmental Stages

作　　者：温晓蕾[1] 吉志新[1] 王长青[1] 武延超 李春华[1] 董洪平[1] 

机构地区：[1]河北科技师范学院,河北秦皇岛066000 [2]河北省馆陶县农业局,河北馆陶057750

出　　处：《安徽农业科学》2010年第3期1160-1162,1190,共4页Journal of Anhui Agricultural Sciences

基　　金：河北省教委资助项目(2003308);秦皇岛市科委资助项目

摘　　要：[目的]对黑色型黄粉虫各虫态及龄期的酯酶和过氧化物酶同工酶进行研究,为其物种鉴定和大规模饲养提供理论依据。[方法]采用聚丙烯酰胺凝胶电泳法分析不同发育时期的黑色型黄粉虫的酯酶和过氧化物酶同工酶谱。[结果]黑色型黄粉虫各虫态酯酶同工酶和过氧化物酶同工酶间存在着较大的差异性,并且不同龄期的幼虫之间和不同性别成虫之间也存在差异。[结论]不同发育时期黑色型黄粉虫的酯酶和过氧化物酶同工酶存在差异,表现出不同的生理特征。[ Objective ] The research aimed to study esterase isozyme and peroxidase isozyme of black worm Tenebrio molitor Linnaeus in different developmental stages or instars, and thus provide a theoretical basis for species identification and large-scale fanning. [ Method ] The polyacrylamide gel electrophoresis（PAGE） technology was used to analyze the esterase isozymes and peroxidase isozymes of Tenebrio molitor Linnaeus in different developmental stages. [ Result] The results showed that the esterase isozyme and peroxidase isozyme of black worm Tenebrio molitor Linnaeus had big difference in different developmental stages. Moreover, the isozyme of larvaes in different instars and adults with different sex were also different. [ Conclusion] The esterase isozyme and peroxidase isozyme of black worm Tenebrio molitor Linnaeus are different at various developmental stages, and its corresponding physiological characteristics also change.

关 键 词：黄粉虫 酯酶 过氧化物酶 电泳 

分 类 号：Q965[生物学—昆虫学]