尼克酰胺与核黄素提高红串红球菌USTB-03对4,6-二甲基二苯并噻吩脱硫活性研究  

Increasing biodesulfurization activity of 4,6-dimethyldibenzothiophene of Rhodococcus erythropolis USTB-03 using nicotinamide and riboflavin

作　　者：倪锦俊[1] 翟羽佳[1] 刘玉凤[1] 高盼盼[1] 宋亚琼[1] 闫海[1] 

机构地区：[1]北京科技大学应用科学学院生物科学与技术系,北京100083

出　　处：《环境工程学报》2010年第1期226-230,共5页Chinese Journal of Environmental Engineering

基　　金：国家自然科学基金资助项目(20777008);北京科技大学科技创新项目(2007010)

摘　　要：主要考察了尼克酰胺和核黄素提高红串红球菌USTB-03对4,6-二甲基二苯并噻吩(4,6-DMDBT)脱硫活性的效应。研究结果表明,在培养基中添加初始浓度为10 mmol/L的尼克酰胺,脱硫比活性由原来302.4 mmol 4,6-DMDBT/(kg细胞干重·h)增加到476.4 mmol 4,6-DMDBT/(kg细胞干重·h),提高了57.5%。添加初始浓度为25μmol/L的核黄素,脱硫比活性增加到了465.8 mmol 4,6-DMDBT/(kg细胞干重·h),提高了54%。无论是采用微量的尼克酰胺或核黄素都可以大幅度提高红串红球菌USTB-03对4,6-DMDBT的脱硫活性,在此研究领域尚未发现有研究报道。尼克酰胺和核黄素分别是NAD和FMN的主要成分,可以促进脱硫关键辅酶FMNH_2的生物合成,因此,大幅度提高了红串红球菌USTB-03对4,6-DMDBT的脱硫活性。Effects of nicotinamide and riboflavin on the sulfur specific activity （SA） of 4,6-dimethyl- dibenzothiophene （4,6-DMDBT） biodesulfurization by Rhodococcus erythropolis USTB-03 were investigated. When R. erythropolis USTB-03 was grown on medium containing nicotinamide of 10 mmol/L, SA was raised 57.5% from 302.4 to 476.4 mmol 4,6-DMDBT/（kg-dry cells.h）. When R. erythropolis USTB-03 was grown on medium containing riboflavin of 25μmol/L, SA increased to 465.8 mmol 4,6-DMDBT/（ kg-dry cells.h） , which was 54% higher than that of control. Both nicotinamide and riboflavin were found to enhance expression of SA, which was not previously reported. It is suggested that the biological synthesis of FMNH2 , an essential coenzyme for the activities of biodesulfurization enzyme, might be enhanced by nicotinamide and riboflavin, which was responsible for the increased SA of R. erythropolis USTB-03.

关 键 词：尼克酰胺 核黄素 4 6-二甲基二苯并噻吩 红串红球菌USTB-03 生物脱硫 

分 类 号：X17[环境科学与工程—环境科学]