RhoA表达与非小细胞肺癌侵袭转移及预后的关系  

The Correlation between the Expression of RhoA and the Invasion and Metastasis of Tumor and Its Impaction on the Prognosis in Human Non-small Cell Lung Cancer

作　　者：袁凯[1] 袁云峰[2] 汪灏[2] 郑如恒[2] 钱成[2] 

机构地区：[1]南京医科大学附属常州第二人民医院心胸外科,江苏常州213003 [2]复旦大学附属中山医院胸外科,上海200032

出　　处：《中国临床医学》2009年第6期831-834,共4页Chinese Journal of Clinical Medicine

基　　金：复旦大学青年科学基金

摘　　要：目的:探讨小G蛋白家族RhoA蛋白与非小细胞肺癌(NSCLC)侵袭转移的关系及对预后的影响。方法:采用组织芯片技术及免疫组化Envision二步法检测140例NSCLC手术标本中RhoA及MMP-9、E-cadherin的表达。结果:140例NSCLC标本中RhoA蛋白在66例(47%)中有高表达,其在肺癌中的表达水平与病理类型、淋巴结转移及TNM分期密切相关,差异有统计学意义(P<0.05),而与患者年龄、性别、肿瘤位置、细胞分化程度无关(P>0.05)。RhoA高表达伴有MMP-9高表达及E-cadherin低表达,差异有统计学意义(P<0.05)。Kaplan-Meier单因素生存分析显示RhoA高表达为NSCLC患者预后的不良因素(P<0.05)。结论:RhoA蛋白在NSCLC的侵袭转移中起重要作用。Objective：To explore the correlation between the expression of the small G protein RhoA and the invasion and metastasis of non-small cell lung cancer （NSCLC） and the impaction of RhoA on the prognosis. Methods：Immunohistologic expres sion of RhoA was assessed in a tissue microarray of 140 operable NSCLC samples using the EnVision IHC procedure. Results： High RhoA expression was noted in 66/140（47 % ） of the 140 cases. A significant （P〈0.05） was found between the expression of RhoA and the pathological type, lympha node metastasis and the TNM stage, while there was no correlation with age, sex, location, differentiation and the tumor size （P〉0. 05）. High RhoA expression correlated well with high MMP-9 expression and low E-eadherin expression, and it reached statistical significance （P〈0.05）. Using Kaplan-Meier method, tumor RhoA expression was found a statistically significant adverse prognostic factor in terms of the overall survival （P = 0. 007）. Conclusions： RhoA protein plays an important role in the invasion and metastasis of NSCLC.

关 键 词：RHOA 基质金属蛋白酶-9 E钙粘蛋白 非小细胞肺癌 组织芯片 免疫组化 

分 类 号：R734.2[医药卫生—肿瘤]