蛇毒神经生长因子对大鼠脑缺血再灌注损伤后神经细胞GAP-43表达的影响  

Effects of Venom Nerve Growth Factor on GAP-43 of Nerve Cell Following Cerebal Ischemia/reperfusion in Rat

作　　者：石胜良[1] 张跃龄[1] 陈仕检[1] 毕桂南[1] 黎彬如[2] 刘唐威[1] 

机构地区：[1]广西医科大学第一附属医院神经内科,广西南宁市530021 [2]广西壮族自治区民族医院神经内科,广西南宁市530021

出　　处：《中国康复理论与实践》2009年第12期1104-1107,F0003,共5页Chinese Journal of Rehabilitation Theory and Practice

基　　金：广西自然科学基金(桂科自0728133);广西医科大学博士后流动站基金(20702011046)

摘　　要：目的探讨蛇毒神经生长因子(vNGF)对大鼠脑缺血再灌注的保护作用。方法选用体重220～280g的健康雄性Wistar大鼠,分为假手术组、对照组及vNGF组(再分为25U、50U、100U3个剂量组)。所有大鼠侧脑室注药7d后处死。大鼠模型分别观察:按Longa法进行神经功能评分;免疫组化法检测GAP-43的表达。结果神经功能评分:假手术组神经功能评分为0分,其余各组均出现神经功能缺损,vNGF组大鼠评分低于对照组(P<0.05)。免疫组化:除假手术组外各组GAP-43均有表达。vNGF组的表达较对照组高(P<0.05)。vNGF组25U组GAP-43表达增加,50U组表达继续增高,100U组表达最高。结论在大鼠大脑中动脉缺血再灌注损伤后,vNGF能增强GAP-43的表达,对神经细胞有明显的保护作用。Objective To investigate the cerebral ischemia/reperfusion protection mechanism of viper venom nerve growth factor（vNGF） by the change of expression of growth associated protein-43 （GAP-43） and neurological function.Methods 45 adult male Wistar rats （weight 220～280 g） were divided randomly into 3 groups：sham group（S,n=9）,balanced salt solution group （BSS,n=9） and venom nerve growth factor group （vNGF,n=27）.Each group was observed for 7 days.vNGF group was divided into 25 U,50 U and 100 U subgroups respectively.The following indexes in 3 groups were observed respectively：neurologic deficits and the expression of GAP-43 （immunohistochemistry method）.Results Neurological function：The scores of neurological function was 0 in S group.The neurological deficits score was lower at the same time in vNGF group than that in BSS group （P〈0.05）.Immunohistochemistry：GAP-43 expressed in both BSS group and vNGF group.The expression of GAP-43 in vNGF group increased in 25 U,and to maximum in 100 U.The expression of GAP-43 in BSS group was significantly lower than in vNGF group （P〈0.05）.Conclusion vNGF can effectively enhance and prolong the expression of GAP-43,increase the survival rats of nerve cells,and has the protection effect on nerve cells after cerebral ischemia injured.

关 键 词：大鼠 脑缺血再灌注 蛇毒神经生长因子 神经细胞生长相关蛋白-43(GAP-43) 

分 类 号：R743.32[医药卫生—神经病学与精神病学]