洋葱根尖细胞有丝分裂制备方法改进研究  

Study on the Improvement of Microscopic Observation Experimental Method for Mitosis of Onion Root Tip

作　　者：马云[1] 王勤[1] 王启钊[1] 姚瑾[1] 徐源[1] 王军芳[1] 

机构地区：[1]信阳师范学院生命科学学院,河南信阳464000

出　　处：《新乡学院学报》2009年第5期54-56,共3页Journal of Xinxiang University

基　　金：河南省自然科学基金项目(092300410010);河南省教育厅高校青年骨干教师资助计划项目;信阳师范学院青年骨干教师资助计划项目(2006BAD04A16)

摘　　要：以洋葱根尖为材料,采取对比法比较观察效果,确定了材料的加热处理、染液的选用、取材时间、硝酸铅溶液处理作为改进内容。结果表明:对确定的材料加热处理、选用改良的品红、在10∶00时取材均使有丝分裂相更清晰,便于观察,而用硝酸铅溶液处理,处理时间越长,有丝分裂指数越小。This paper onion root tip as the material, compared to observe the effect of contrast to determine the materials, heat treatment, the choice of dye liquor, drawing time, lead nitrate solution treatment as the improvements. The results showed that： in determining the heat treatment of materials, selection of improved magenta, at 10 ： 00 am drawn to the mitotic phase are clearer, easy to observe, while the use of lead nitrate solution, handling the longer the mitotic index lower.

关 键 词：洋葱根尖 有丝分裂 制备方法改进 

分 类 号：Q94-3[生物学—植物学]