牛粪挥发性成分的毛细管气相色谱分析  

Analysis of Volatile Components in Ox Feces by Capillary Gas Chromatography

作　　者：丁强[1] 邓越[1] 孙玉芳[1] 黄爱今[1] 孙亦梁[1] 

机构地区：[1]北京大学化学与分子工程学院

出　　处：《北京大学学报（自然科学版）》1998年第6期720-725,共6页Acta Scientiarum Naturalium Universitatis Pekinensis

摘　　要：利用同时蒸馏萃取法（ＳＤＥ）提取了有治疗恶疮、霍乱效果的牛粪挥发性成分。用毛细管ＧＣ－ＭＳ联用法分离并初步鉴定了５０种组分，占总峰面积的７０．９２％，进一步用程序升温保留指数和标准样品联合确证了其中的２４种化合物，占总峰面积的３４．６８％，与Ｓａｄｔｌｅｒ保留指数库文献数据对照又确证了另外８种化合物，占总峰面积的６．０９％。其中含量较高的组分是己醛（６．２０％），三硫化二甲基（５．４８％），二硫化二甲基（４．５５％），壬醛（４．３５％），二甲基四磺酸酐（３．９６％），辛醛（３．６１％），庚醛（３．２２％）。从化合物的种类来看，主要为：醛类（１４个），醇类（３个），酮类（１２个），酸类（２个），它们共占总峰面积的４４．５４％。According to the traditional Chinese medicine,it was reported that the volatiles of the ox feces are effective in curing many illness.We collected the volatiles of ox feces by using a simultaneous distillation and extraction (SDE) equipment.The chemical composition of the enriched volatiles was examined by means of capillary gas chromatography and combined gas chromatography mass spectrometry.50 constituents of the volatiles were preliminarily identified by GC MS,which amounted to 70.92% of the total peak areas.Of these compounds,24 were further verified by measuring their temperature programmed retention indexes or retention times on SE 54 and PEG 20M columns and making comparison with those of the corresponding authentic samples,which amounted to 34.68% of the total peak areas.Another 8 constituents of the volatiles were verified by making comparison with the data in The Sadtler Standard Gas Chromatography Retention Index Library,which amounted to 6.09% of the total peak areas.The main constituents of the volatiles are hexanal (6.20%),dimethyl trisulfide (5.48%),dimethyl disulfide (4.55%),nonanal (4.35%),dimethyl tetrasulphide (3.96%),octanal (3.61%),heptanal (3.22%).The compound classes consist of aldehydes (14),alcohols (3),ketones (12) and acids (2).

关 键 词：牛粪 挥发性成分 中药 毛细管气相色谱 SDE 

分 类 号：R284.1[医药卫生—中药学] O657.71[医药卫生—中医学]