Elf-1基因在急性髓细胞性白血病中的表达情况  

The feature of Elf-1 gene expression in acute myeloid leukemia

作　　者：沈琦[1] 郑海涛[1] 陈少华[1] 杨力建[1] 李扬秋[1,2] 

机构地区：[1]暨南大学医学院血液病研究所,广东广州510632 [2]暨南大学再生医学教育部重点实验室,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2009年第6期581-584,共4页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：国家自然科学基金项目(30871091)

摘　　要：目的:建立实时定量PCR检测Elf-1基因表达水平的方法,了解急性髓细胞性白血病(AML)患者外周血Elf-1基因表达水平。方法:采用SYBR GreenⅠ荧光定量PCR和相对定量分析法检测33例AML患者:M2型17例、M3型6例、M5型10例和20例健康人外周血的单核细胞的Elf-1表达情况,以β2微球蛋白基因(β2M)作为内参,采用公式2-ΔCt×100%计算Elf-1基因表达水平。结果:AML组Elf-1表达水平(13.518±19.197)%明显高于健康对照组(2.044±1.321)%(P<0.01),不同AML亚型之间Elf-1的表达水平没有显著性差异(P=0.52),但各亚型AML的Elf-1的表达水平均与健康对照组比较均有显著性差异(P<0.01)。结论:建立SYBR GreenⅠ荧光实时定量PCR分析外周血Elf-1表达水平的方法,检测急性髓细胞性白血病Elf-1基因高表达情况。Aim：To establish a real-time PCR technique for detection and quantify of Elf-1 gene expression and to investigate the expression level of Elf-1 gene in patients with acute myeloid leukemia （AML）. Methods： Real-time PCR with SYBR Green I technique was used for detecting Elf-1 expression level in peripheral blood mononuclear cells of 33 patients with AML （including 17 cases with M2, 6 cases with M3, 10 cases with M5 and 20 healthy individuals）. β2-microglobulin gene （β2M） was used as an endogenous reference. Relative changes in Elf-1 expression level were used by the 2-△Ct×100% method. Results： The expression level of Elf-1 in 33 cases with AML（ 13. 518 ± 19. 197% ） was significant higher than those in the healthy control （2. 044 ± 1.321% ） （P 〈0. 01 ）. The expression level of Elf-1 had not significant difference among the three subtypes （P = 0. 52）. However, by comparing with the healthy control, the expression level of Elf-1 had significant difference in all of three subtypes （ P 〈 0. 01 ）. Conclusion：The SYBR Green I real-time technique for quantitative detection of Elf-1 expression levels was established. And the overexpression of Elf-1 gene in AML was detected.

关 键 词：Elf-1基因 实时定量PCR 急性髓细胞性白血病(AML) T细胞免疫缺陷 TCRζ链 

分 类 号：R733.71[医药卫生—肿瘤] R392.11[医药卫生—临床医学]