缺氧后小鼠脑匀浆提取液对儿童MSCs分化为神经细胞的影响  

Homogeneous extract of hypoxia mice brain induce child's MSCs to neuron-like cells

作　　者：康颖[1] 罗向阳[2] 黄云燕[1] 李栋方[2] 何展文[2] 岑丹阳[2] 

机构地区：[1]广东药学院临床医学院儿科教研室,广东广州510310 [2]中山大学附属第二医院儿科,广东广州510120

出　　处：《暨南大学学报（自然科学与医学版）》2009年第6期664-667,共4页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：广东省自然科学基金(07001590)

摘　　要：目的:观察缺氧后不同时段小鼠脑匀浆提取液对儿童骨髓间充质干细胞(MSCs)分化为神经元样细胞的影响,为筛选MSCs的最佳移植时间提供依据。方法:制作缺氧小鼠模型;并制取缺氧不同时间段脑匀浆提取液;诱导儿童MSCs;比较MSCs分化为神经元样细胞的阳性率。结果:缺氧后1、4、7、10、13 d小鼠脑匀浆提取液均可将MSCs诱导成神经元样细胞,经免疫学鉴定NES染色强阳性;但各组阳性率不同,以缺氧10 d后阳性率(21.02±3.38)%最高,与其他各组比较有统计学差异(P<0.05)。结论:缺氧后小鼠脑匀浆提取液可诱导儿童MSCs分化为神经元样细胞,缺氧后10 d的分化率最高。Aim：To investigate the effects of homogeneous extract of hypoxia mice brain induce the marrow mesenchyma stem cells （MSCs） of child to neuron-like cells, to select the best time when MSCs are transplanted in vitro. Methods： Established animal model of anoxic mouse and refined the extract of brain organization, induced MSCs to neuron-like ceils and identificated the neuron-like ceils immunochemically and compared the different positive rate. Results： MSCs can be induced to neuron-like ceils which show positive when staining by NSE, and the highest positive rate is A10 （ 21.02 ± 3.38 ） %, which occured 10 days later after hypoxia. Conclusion：MSCs can be induced to neuron-like cells by extract of hypoxia mice brain, and the 10th day later after hypoxia is the best time.

关 键 词：缺氧 脑匀浆诱导液 骨髓间充质干细胞 神经元样细胞 

分 类 号：R748[医药卫生—神经病学与精神病学]