川芎嗪对脑缺血—再灌注大鼠神经功能及神经电生理的影响  

Neuroelectrophysiologic effect of ligustrazine in cerebral ischemia-reperfusion injury rats

作　　者：方征宇[1] 熊亮[1] 黄杰[1] 杨唐柱[1] 马玉娟[1] 尤春景[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院,武汉430030

出　　处：《山东医药》2010年第2期19-20,共2页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(30801220)

摘　　要：目的观察腹腔注射川芎嗪对脑缺血—再灌注大鼠神经功能及神经电生理的影响。方法将20只大鼠随机分为A、B组,制作大鼠大脑中动脉栓塞模型后,分别腹腔注射生理盐水及川芎嗪80 mg/kg,2次/d,连用7 d。采用双盲法行大鼠神经功能评分,采用TTC染色法测量大鼠脑梗死灶体积,并行运动诱发电位检测。结果A组神经功能评分为(3.31±0.23)分,脑梗死灶体积为(45.63±12.75)mm3,运动阈值为52.67%±8.75%,B组分别为(2.30±0.15)分、(26.59±7.64)mm3、34.29%±5.18%,P均<0.05。损伤后的运动阈值与脑梗死灶体积呈正相关(r=0.68,P<0.05)。结论川芎嗪可减小缺血—再灌注损伤脑组织梗死灶体积,提高皮质兴奋性。Objective To observe the effects of ligustrazine on neurvous function and cortical excitability of rats with cerebral ischemia-reperfusion injury. Methods 50 Healthy male SD rats were randomly divided into 5 groups ： sham operation group, model group, low-dose lignstrazine group （20 mg/kg）, middle-dose ligustrazine group （40 mg/kg）, high-dose lignstrazine group （80 mg/kg）. All medications were given intraperitoneally twice a day for 7 days. Motor threshold （ MT）, motor evoked potential （MEP） and the scores of neurological deficits were evaluated. Results 7 days after MCAO reperfusion, the scores in middle and high-dose ligustrazine group were significantly lower than those of the model group. The infarct volumes of high-dose lignstrazine group were significantly smaller than those of the model group （ P 〈 0.05 ）. The post-injury MT of the model group was significantly higher than the preinjnry level. The post-injury MT of the high-dose lignstrazine group was significantly lower than that of the model group. Positive linear correlation was found between post-in- jury motor threshold and infarct volume. Latency of MEP in the model group and lignstrazine group was significantly prolonged compared with that of the previous. Compared with the pre-injury level, there was no significantly difference of the post-injury latency of MEP in model group and ligustrazine group （ P 〉 0.05 ）. Conclusion Ligustrazine can protect neuro effect on MACO reperfusion, reduce the infarct volumes, increase the cortex excitability.

关 键 词：川芎嗪 脑缺血 再灌注损伤 大鼠 诱发电位 运动阈值 

分 类 号：R364.2[医药卫生—病理学]