肝癌相关抗原kinectin基因重组蛋白致敏的树突状细胞对T细胞增殖活化的影响  

Effect of DC sensitized with kinectin proteinon on T cell proliferation activity

作　　者：赵飞兰[1] 晁耐霞[1] 薛慧琴[1] 吴春林[1] 林永达[1] 罗彬[1] 陈芳[1] 谢小薰[1] 罗国容[1] 

机构地区：[1]广西医科大学,南宁530021

出　　处：《山东医药》2010年第2期21-23,共3页Shandong Medical Journal

基　　金：教育部高校博士点专项基金资助项目(20050598006);广西教育厅普通课题资助项目(200710LX025);广西高校博士研究生创新课题资助项目(2009105981001D28)

摘　　要：目的观察肝癌相关抗原kinectin基因重组蛋白(kinectin-MBP)致敏的树突状细胞(DC)对T细胞增殖活化能力的影响。方法从正常人外周血中分离单个核细胞,用rhGM-CSF、rhIL-4联合诱导扩增DC;用kinectin-MBP(kinectin-MBP组)、麦芽糖结合蛋白(MBP组)分别致敏DC,对照组不处理。采用ELISA法检测细胞上清液中的IL-12、IFN-γ,MTT法检测各组DC刺激自体T淋巴细胞增殖的能力。结果kinectin-MBP组上清液中IL-12、IFN-γ含量分别为(615.32±7.93)、(544.28±7.17)pg/ml,MBP组分别为(382.13±5.21)、(308.46±6.44)pg/ml对照组组分别为(299.40±10.22)、(228.03±6.70)pg/ml,三组相比,P均<0.05。kinectin-MBP组T淋巴细胞增殖指数为53.14±0.62,MBP组为22.14±0.31,对照组为10.86±0.65,三组相比,P均<0.001。结论体外诱导扩增的DC经kinectin-MBP致敏后具有很强的刺激自体T细胞增殖的能力。Objective To observe the effect of dendritic eel/s（DC） senitized with recombinant protein kinectin sensitized on T cell proliferation activity. Methods The DC was induced and expanded from normal human peripheral blood mononuelear ceils（PBMCs） in a culture medium supplemented with rhGM-CSF and IL-4. For identifying the cultured DC, light and electronic microscopes were used to examine the morphologie characteristics of cultured ceils. Cultured DC were impulsed by maltose binding protein （MBP） and Kineetin-MBP fusion protein, and then co-cultured with autologous T cells. The proliferation activity of co-cultured T ceils was detected by MTI＇ method. And the concentrations of IL-12（ CIL- 12） and IFN-γ（CIFN-3,） in culture medium supernatants were mensurated by ELISA. Results After induced by rhGM- CSF and IL-4 for 7 days, PBMCs presented typical morphologic characteristics of DC. In Kinectin-MBP fusion protein treated group, comparing with the other two groups, the proliferation activity of co-cultured T ceils was enhanced remarkably（P 〈 0.05 ） ; the CIL-12 and CIFN-γ in culture medium supernatants were raised significantly（ P 〈 0.05 ）. Conclusion The DC sensitized by kineetin-MBP fusion protein can obtain the capability of stimulating autologous T cell proliferation.

关 键 词：树突状细胞 kinectin基因 重组蛋白 T细胞 麦芽糖结合蛋白 

分 类 号：R735.7[医药卫生—肿瘤]