白车轴草化学成分的分离与鉴定  

Isolation and identification of whole plant of chemical constituents from Trifolium repens L.

作　　者：孙琳[1] 李占林[1] 彭静波[1] 韩国华[1] 华会明[1] 

机构地区：[1]沈阳药科大学中药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2010年第1期40-43,共4页Journal of Shenyang Pharmaceutical University

摘　　要：目的为白车轴草(Trifolium repensL.)的药效物质基础提供依据。方法利用制备薄层、反复硅胶、Sephadex LH-20、开放ODS柱色谱等方法进行分离纯化,根据理化性质及波谱分析对分离得到的化合物进行结构鉴定。结果分离得到10个化合物,分别鉴定为伞形花内酯(umbelliferon,1)、芒柄花素(formononetin,2)、水杨酸(salicylic acid,3)、双白瑞香素(daphnoretin,4)、4′-methoxy-coumestrol(5)、trifoliol(6)、美迪紫檀素(medicarpin,7)、环阿尔廷-25-烯-3β,24ξ-二醇(cycloart-25-en-3β,24ξ-diol,8)、3β-hydroxy-7α-methoxy-24β-ethyl-cholest-5-ene(9)、soyasapogenol B(10)。结论化合物8、9为首次从车轴草属植物中分离得到,化合物1、3、4为首次从白车轴草中分离得到。Objective To isolate and identify the chemical constituents of the 95% ethanol extract of the whole plant of Trifolium repens L.Methods Compounds were isolated by various kinds of column chromatography on silica gel,Sephadex LH-20 and ODS,and their structures were identified by the physico-chemical characters and the spectral analysis.Results Ten compounds were isolated and their structures were identified as umbelliferon（1）,formononetin（2）,salicylic acid（3）,daphnoretin（4）,4′-methyoxycoumestrol（5）,trifoliol（6）,medicarpin（7）,cycloart-25-en-3β,24ξ-diol（8）,3β-hydroxy-7α-methoxy-24β-ethyl-cholest-5-ene（9）,soyasapogenol B（10）,respectively.Conclusions Compounds 8 and 9 are isolated from Trifolium genus for the first time,and compounds 1,3,and 4 are isolated from Trifolium repens L.for the first time.

关 键 词：白车轴草 化学成分 环阿尔廷-25-烯-3β 24ξ-二醇 3β-hydroxy-7-αmethoxy-24-βethyl-cholest-5-ene 结构鉴定 

分 类 号：R914[医药卫生—药物化学] R284[医药卫生—药学]