锯叶棕提取物增强多烯紫杉醇抗MM细胞增殖作用  

Serenoa Repens Extract enhance docetaxel anti- MM cell proliferation function

作　　者：车玉琴[1] 张立德[1] 

机构地区：[1]辽宁中医药大学,辽宁沈阳110032

出　　处：《实用中医内科杂志》2010年第1期36-37,共2页Journal of Practical Traditional Chinese Internal Medicine

摘　　要：[目的]探讨锯叶棕提取物对多烯紫杉醇抗MM细胞增殖的影响。[方法]将体外培养的U266和PRMI8226细胞与0-2μL/mL的不同浓度锯叶棕提取物共孵育,应用MTT比色法测定细胞增殖活性;同时应用台盼蓝不相容实验检测锯叶棕提取物与多烯紫杉醇单独使用或合并使用时的细胞成活率。[结果]锯叶棕提取物对U266细胞与RP-M18226细胞的增殖具有明显的抑制作用,抑制生长50%(ED_(50S))的有效剂量分别为1.2和0.7μL/mL;U266细胞用锯叶棕提取物或多烯紫杉醇预处理,其细胞成活率分别为79.7%或90.1%,两者联合应用细胞成活率为51.6%(P<0.001);RPMI8226细胞用锯叶棕提取物或多烯紫杉醇预处理,其细胞成活率分别为73.7%或80.1%,两者联合应用细胞成活率为55.6%(P<0.001)。[结论]锯叶棕提取物抑制MM细胞增殖并增强多烯紫杉醇抗MM细胞增殖作用。[ Objective] Discuss serenoa repens extract to docetaxel anti -MM cell proliferation influence. [Methods] U266 and PRMI8226 cells cultured in vitro with different concentrations 0 -2μL/mL serenoa repens extracts were incubated together, using MTT colorimetry assay cell proliferation activity, at the same time using trypan blue exclusion test detect the cell survival rate when serenoa repens extract used alone or in combination with docetaxel. [ Results ] Serenoa repens extract significantly inhibited the proliferation of U266 and RPMI8226 cells, inhibit the growth of its 50% （ED505） the effective dose were 1.2 and 0.7μL/mL; pretreatment of U266 cells used serenoa repens extract or docetaxel, the cell survival rate was 79.7 % or 90. 1%, combination of both the cell survival rate was 51.6% （P 〈 0. 001 ）; pretreatment of RPMI8226 cells used serenoa repens extract or docetaxel, the cell survival rate was 73.7% or 80.1% , combination of both the cell survival rate was 55.6% （P 〈0. 001 ）. [ Conclusion] Serenoa repens extract inhibited MM cell proliferation and enhanced docetaxel anti -MM cell proliferation.

关 键 词：多发性骨髓瘤 锯叶棕提取物 细胞增殖 多烯紫杉醇 

分 类 号：R-33[医药卫生]