胎盘生长因子在肝癌组织中的表达及意义  

Expression and significance of placenta growth factor in hepatocellular carcinoma

作　　者：林胜弟[1] 潘陈为[1] 金玲湘[1] 黄智铭[2] 黄卡特[2] 陈永平[2] 

机构地区：[1]温州医学院附属第二医院,325000 [2]温州医学院附属第一医院,32500

出　　处：《实用医学杂志》2010年第1期42-44,共3页The Journal of Practical Medicine

基　　金：浙江省自然基金资助项目(编号:Y207464);温州市科学技术局资助项目(编号:Y20060138)

摘　　要：目的:研究胎盘生长因子(PLGF)在肝癌组织中的表达,探讨其与肝癌的联系。方法:收集标本:肝细胞肝癌组织49例及正常肝组织8例,用免疫组化方法和逆转录聚合酶链式反应(RT-PCR)技术检测PLGF的表达。结果:PLGF蛋白和mRNA在肝癌组织有高水平表达,与正常肝组织比较差异有显著性意义(P<0.01)。结论:PLGF与肝细胞肝癌的发生有一定的关联,可能在肝肿瘤血管新生过程中起重要作用。Objective To investigate the expression of placenta growth factor （PLGF）in human hepatocellular carcinoma （HCC）, and explore its correlation with HCC. Methods Expression of PLGF mRNA and protein in surgical specimens which including 49 hepatocellular carcinoma （HCC） tissues and 8 normal hepatic tissues were detected by immunohistochemistry and reverse transcriptase polymerase chain reaction. The relative value of their expression were examined with absorhancy or optical density analysis. Results There were a high level expressions of PLGF mRNA and protein in the HCC,and have significant differences between the HCC and the normal hepatic tissues （P 〈 0.01）. Conclusion PLGF is relate to HCC, and may play an important role in the process of vessel neogenesis in HCC.

关 键 词：肝肿瘤 胎盘生长因子 逆转录聚合酶链式反应 

分 类 号：R735.7[医药卫生—肿瘤]