超声波对荧光原位杂交技术检测氨氧化菌的影响  

Effect of Ultrasonic Waves on Detection of Ammonia-oxidizing Bacteria by Fluorescence In-situ Hybridization Technology

作　　者：张可方[1] 罗思音[1] 荣宏伟[1] 张立秋[1] 

机构地区：[1]广州大学土木工程学院,广东广州510006

出　　处：《中国给水排水》2010年第2期89-92,共4页China Water & Wastewater

基　　金：国家自然科学基金资助项目(50878058);广东省自然科学基金资助项目(8151009101000015);广州市教育局科技计划(62018)

摘　　要：介绍了荧光原位杂交技术的基本原理和操作步骤。在采用荧光原位杂交技术检测氨氧化菌时必须经过预处理,而超声波对细胞的破碎是预处理中的关键步骤。试验通过比较预处理中超声波的三个强度对荧光原位杂交技术检测SBR反应器中氨氧化菌的效果,筛选出最佳的细胞破碎强度。结果显示,经过强度为150W超声破碎后,在荧光显微镜下观察,无论是丰度还是亮度方面,效果均比经过强度为100W或200W破碎后的好。The basic principles and operation steps of the fluorescence in-situ hybridization technology are introduced. Before using the fluorescence in-situ hybridization technology for detecting ammonia-oxidizing bacteria, the pretreatement must be carried out, and the uhrasonie cell disruption is a crucial step in the pretreatment. The effect of three ultrasonic intensity levels on detection of ammonia-oxidizing bacteria in SBR reactor by fluorescence in-situ hybridization technology was compared to achieve the optimal intensity for cell disruption. The results show that after 150 W ultrasonication, the effect observed under fluorescent microscope, whether abundance or brightness, is better than that after 100 W or 200 W ultrasonication.

关 键 词：荧光原位杂交技术 氨氧化细菌 超声波处理 

分 类 号：X703.1[环境科学与工程—环境工程]