前列腺特异性抗原(PSA)免疫放射分析及临床应用  

IMMUNORADIOMETRIC ASSAY AND ITS CLINICAL APPLICATION FOR PROSTATE SPECIFIC ANTIGEN(PSA)

作　　者：崔素珍[1] 王玉肖[1] 刘一兵[1] 

机构地区：[1]中国原子能科学研究院同位素研究所

出　　处：《同位素》1998年第3期149-152,共4页Journal of Isotopes

摘　　要：以１２５Ｉ－抗ＰＳＡ单克隆抗体为示踪剂，抗ＰＳＡ多克隆抗体为固相包被抗体，采用两步法，建立了ＰＳＡ免疫放射分析方法。其灵敏度为０．５μｇ／Ｌ，批内变异系数为１．１％—３．７％，批间变异系数为１．４％—７．０％，平均回收率为９７．５％。测定了１００名６３岁以下健康男性血清ＰＳＡ含量，正常参考值＜３．８μｇ／Ｌ；２０例前列腺良性疾病患者血清ＰＳＡ含量为２４．３±１３．８μｇ／Ｌ；９例前列腺癌患者血清ＰＳＡ含量为８７．０±５６．３μｇ／Ｌ。本法与放射免疫分析法血清ＰＳＡ测定值相关性良好，ｒ＝０．９２４（ｎ＝１０９），与ＰＳＡ酶免疫分析法（ＭａｘｉｍＢｉｏｔｅｃｈ）相关系数为０．７８１（ｎ＝２９）。The immunoradiometric assay for PSA is developed. The assay involves both monoclonal antibody and polyclonal antibody. The monoclonal antibody is labeled with 125 I,and the polyclonal antibody is coated on the plastic tubes. The sensitivity of assay is 0.5 μg/L, the CVs are 1.1%-3.7% for the intra assay and 1.4%-7.0% for the inter assay and the average recovery is 97.5%.The reference interval established from 100 normal men are 0-3.8 μg/L. The clinical utility of PSA is evaluated in 20 cases of benign prostatic diseases and in 9 cases of prostatic cancers. Their data of sera PSA are 24.3±13.8 μg/L and 87.0±56.3 μg/L,respectively. The correlation coefficient comparing with the PSA RIA is 0.924( n =109), and the correlation coefficient comparing with the PSA EIA(Maxim Biotech) is 0.781( n =29).

关 键 词：特异性抗原 PSA 前列腺癌 免疫放射分析 

分 类 号：R737.25[医药卫生—肿瘤] R730.45[医药卫生—临床医学]