人肝癌、癌旁组织中AFPmRNA的差异性分析  

Analysis of AFPmRNA in Human Hepatoma, Paratumor Tissues

作　　者：贺平[1] 刘彬彬[1] 叶胜龙[1] 汤钊猷[1] 

机构地区：[1]上海医科大学中山医院,肝癌研究所,上海200032

出　　处：《中国肿瘤生物治疗杂志》1998年第3期163-166,共4页Chinese Journal of Cancer Biotherapy

基　　金：国家自然科学青年基金(390050)资助项目

摘　　要：本研究用RT-PCR方法检测52例原发性肝癌(HCC)病人手术标本中AFPmRNA转录水平,结果发现,AFPmRNA在肝癌组织中阳性率为76.9%;有慢性肝病(尤其是中重度肝硬化)的病人癌组织中AFP表达的阳性率可达88.8%,癌旁为69.4%;无慢性肝病(肝硬化)的病人中,其癌组织中AFPmRNA阳性率为50%,癌旁组织中无AFP表达.由此可见,AFP蛋白是由肝癌细胞特异表达的,或由正发生突变的肝细胞表达的.另外,本文还研究了AFRmRNA与肿瘤大小、包膜、肝内转移以及与血清AFP水平的关系.In this study, the RT-PCR method was employed to detect the expression of AFP in mRNA level in tissue samples form 52 patients suffered from hepatocellular carcinoma (HCC) . The results revealed that the positive rate of AFPmRNA was 76.9% in the HCC tumor tissues and 69.4% in the paratumortissues from the HCC patients with severe cirrhosis . Meanwhile, in HCC patients without cirrhosis, the positive rate reached 50% in tumor tissues, but no AFPmRNA expression was found in related paratumor tissues. The study suggested that the AFP protein was specially expressed by hepatoma cells and mutating hepatocytes. The relationships between AFPmRNA and tumoor size, capsule status and tumor metastasis were also demonstrated.

关 键 词：原发性 肝癌 AFP mRNA RT-PCR 肝硬变 

分 类 号：R735.702[医药卫生—肿瘤] R575.206[医药卫生—临床医学]