天麻简单重复序列与天麻素相关性初探  

Primary Study of the Relationship Between the SSR of Gastrodia Elata Blume and the Gastrodin Content

作　　者：王晓丽[1] 宋聚先[1] 李宝忠 何国栋[1] 郑伟耀[1] 

机构地区：[1]贵阳医学院药用植物与生药学教研室,贵阳550004 [2]贵州家诚药业,遵义563000

出　　处：《世界科学技术-中医药现代化》2009年第6期880-884,共5页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：科技部国家"十五"攻关课题(2004BA721A33):天麻种质资源及其评价研究;负责人:王晓丽;贵州省科技厅中药现代化科技产业研究开发专项(黔科合中药专字[2003]51):天麻分子标记辅助保种与产业化开发;负责人:王晓丽;贵州省"十五"攻关课题(黔科合农社字[2001]1125):天麻种质资源分子生物学;负责人:王晓丽

摘　　要：目的:探讨天麻遗传变异与其品质的关系、为天麻优良种质的选育提供科学途径。方法:采用简单重复序列标记技术和高效液相检测不同种质的天麻,进行相关性分析。结果:本研究特异引物产生的特异带型与天麻素含量具有一定的关系,扩增最大片段为520bp最小片段为320bp,出现单上带及双带的样品天麻素含量≥0.20%,出现单下带的样品天麻素含量约为0.20%或<0.20%。结论:本研究初步显示出现高分子量条带类型的样品天麻素含量较高,天麻SSR特异带型与天麻素含量的相关性具有进一步探讨的价值。This work aimed to investigate the relationship between the genetic variation of Gastrodia elata Blume and its species, and try to find a rational approach for the selection of the good species. Simple sequence repeat （SSR） and HPLC were used to test different species of Gastrodia elata Blume and explore their relationship. The results showed that there may exist a certain relationship between the SSP fingerprinting amplified by specific primer pairs and the content of Gastrodia elata Blume. The largest amplified fragment was 520bp, while the smallest 320bp.The species showing simple upper tracts and double tracts had the Gastrodin content of more than 0.20%, while the species showing simple lower tract had the Gastrodin content of 0.20% or even lower. This relationship needs to be further studied.

关 键 词：天麻苷含量 简单重复序列 

分 类 号：R282.71[医药卫生—中药学] R543.506[医药卫生—中医学]