抗癌活性肽-S诱导肝癌细胞凋亡及对细胞周期的调控  

Anti-cancer bioactive peptide-S induced apoptosis of hepatoma cells and cell cycle regulation

作　　者：王朝阳[1] 杨成旺[1] 欧阳晓晖[1] 苏秀兰[1] 

机构地区：[1]内蒙古医学院附属医院,内蒙古呼和浩特010050

出　　处：《中国实验诊断学》2010年第1期1-6,共6页Chinese Journal of Laboratory Diagnosis

基　　金：国家自然科学基金(30860327)

摘　　要：目的探讨抗癌活性肽-S对荷肝癌小鼠肿瘤的抑制作用及其可能机制。方法建立荷肝癌小鼠皮下种植瘤模型,随机分三组,分别给5-Fu,20mg/Kg,抗癌活性肽-S0.4ml/只,对照组给同体积的生理盐水,尾静脉注射,隔日给药连续10天。10天后处死小鼠,计算重量抑瘤率;取瘤组织制备石蜡切片,原位杂交法检测瘤细胞VEGFmRNA表达情况并计算瘤组织微血管密度(MVD),免疫组化法检测瘤组织中Ki67的表达情况;流式细胞仪检测细胞周期及凋亡。结果抗癌活性肽-S具有较好地抑制肝癌细胞增殖生长及血管生成作用,抑制率为49.43%,且对VEGFmRNA及Ki67表达有较显著的抑制作用(P<0.01);流式细胞仪分析显示,抗癌活性肽-S可以阻滞肿瘤细胞停止于S期,促进瘤细胞凋亡。结论抗癌活性肽-S具有抑制肝癌细胞生长增殖的作用,其抗肝癌细胞增殖作用机制为诱导肿瘤细胞凋亡,调控细胞周期。Objective To study the inhibitory effect of anti-cancer bioactive peptide-S （ACBP-S）on the tumor of liver cancer loaded mouse and the possible mechanisim. Methods Established liver cancer loaded mouse mede and divided mice into three groups randomly and gave drugs through tail intravenous injection,the drugs were 5-Fu（20 mg/kg） and ACBP-S 0.4 ml per mouse. NS was given to the control group at the same dosed and in the same way. Every other day for 10 days.Then,executed mice after 10 days and ealculated weight inhibitory rate. Tumor tissues were embedded with paraffin wax and made into slices. Detected the expression of VEGFmRNA and calculated MVD of tumor tissues and the expression of Ki67 protein with immune histochemistry, also deteeded cell cycle distribution and cell apoptosis rate. Results The ACBP-S could inhibit tumor growth and angiogenesis. The inhibitory rate were individually 49.43% .The expression of VEGF mRNA and ki67 were decreased in ACBP-S groups（ P 〈 0.01 ）. ACBP-S could arrest tumor cells at S phase and induce tumor ceils into apoptosis. Conclusion ACBP-S may obviously inhibit the proliferation of liver cancer cells and induce tumor cells into apoptosis.

关 键 词：抗癌活性肽-s 抑瘤作用 肝肿瘤 细胞凋亡 

分 类 号：R735.7[医药卫生—肿瘤]