抑制STAT3表达增加H_2O_2诱导人胶质瘤U251细胞损伤  

Suppression of STAT3 Expression Facilitates Apoptosis Induced by H_2O_2 in Human Glioma U251 Cells

作　　者：苏静[1] 孙际童[1] 刘宁[1] 钟加滕[1] 刘菲[1] 于慧美[1] 康劲松[1] 

机构地区：[1]吉林大学白求恩医学院病理生理学系,吉林长春130021

出　　处：《中国实验诊断学》2010年第1期9-11,共3页Chinese Journal of Laboratory Diagnosis

基　　金：国家自然科学基金(30570687);吉林省科技厅医学专项基金资助项目(200505139);吉林省科技厅资助项目(No.200705373)

摘　　要：目的探讨RNA干涉技术抑制STAT3基因表达对过氧化氢(H2O2)复制的人胶质瘤U251细胞损伤模型的影响。方法转染pSilencer1.0-U6-SiRNA-STAT3重组质粒到U251细胞;Western blot方法观察转染重组质粒对STAT3蛋白表达的影响;MTT法检测细胞生存率,TUNEL法检测细胞凋亡,Western blot检测凋亡相关蛋白BCL-2、BAX表达。结果转染pSilencer1.0-U6-SiRNA-STAT3重组质粒能有效抑制U251细胞STAT3蛋白表达(抑制率>50%);抑制STAT3表达能促进H2O2诱导U251细胞生存率下降,增加细胞凋亡率(P<0.05)。STAT3抑制能促进H2O2作用下U251细胞BCL-2表达降低,BAX表达增加(P<0.05)。结论pSilencer1.0-U6-SiRNA-STAT3可有效抑制U251细胞STAT3的表达,并促进H2O2诱导U251细胞凋亡。Objective To investigate the effect of pSilencer1.0-U6- siRNA-STAT3 on the cell injury of malignant U251 glioma cells induced by hydrogen peroxide （H2O2）.Methods PSilencer1.0-U6-siRNA-STAT3 was transfected into U251 cells. The STAT3 expression in U251 transfected with pSilencer1.0-U6- siRNA-STA＇I3 was detected by Western blot. The viability of U251 cells was measured by M＇IT assay, cell apoptosis was measured by TUNEL assay, the expression of BCL-2 and BAX were determined by West- ern blot. Results Transfection of pSilencerl. 0-U6- siRNA-STAT3 suppressed the expression of STAT3 efficiently in U251 cells （in- hibition ratio 〉 50 % ）. Suppression of STAT3 facilitated the cell death and apoptosis （P 〈 0.05）induced by H202 in U251 cells. In- hibition of STAT3 expression decreased the ratio of BCL-2 and BAX expression with or without H2O2 treatment in U251 cells（ P 〈 0.05）. Conclusion Transfection of pSilencer1.0-U6- siRNA-STAT3 could suppress the expression of STAT3 efficiently and facilitat- ed the apoptosis induced by H2O2 in U251 cells.

关 键 词：胶质瘤 信号转导及转录激活因子3 过氧化氢 凋亡 

分 类 号：R739.4[医药卫生—肿瘤]