CpG ODN对ZP^(121-140)表位合成肽诱导的免疫应答和免疫避孕效应影响  

The effect of CpG ODN on the immune responses and immune-contraception induced by ZP^(121-140) synthetic peptide

作　　者：秦卫兵[1] 王一峰[1] 蒋敏[1] 蒋建伟[2] 苗竹林[1] 欧汝强[1] 

机构地区：[1]广东省计划生育科学技术研究所,广州510600 [2]暨南大学医学院生化教研室,广州510632

出　　处：《中国免疫学杂志》2010年第1期46-50,共5页Chinese Journal of Immunology

基　　金：广东省计划生育委员会科研基金资助项目(No.2004003)

摘　　要：目的:探讨CpGODN对透明带(ZP)抗原表位ZP121-140合成肽诱导的免疫应答和免疫避孕效应影响。方法:应用人工合成ZP2121-140表位肽,与20μgCpGODN或等量完全弗氏佐剂(CFA)混悬后左胫前肌免疫雌性BALB/c小鼠,初次免疫后2、4、6周各加强免疫一次,共免疫四次。在每次加强免疫前和末次免疫后两周断尾取血,分离血清,分析特异性IgG抗体及非特异性细胞因子IFN-γ、TNF-α及IL-10水平;收集小鼠阴道冲洗液,离心取上清,分析特异性IgA抗体水平。免疫小鼠生育实验结束后,取卵巢组织行病理学分析。结果:CpGODN组诱导的特异性IgG和IgA水平较CFA组有增高趋势,但无显著性差异(P>0.05)。CpGODN组诱导的非特异性IFN-γ和TNF-α水平明显高于CFA组(P<0.05);而CpGODN组诱导的IL-10水平显著低于CFA组(P<0.05)。两种佐剂对小鼠的受孕率影响没有显著性差异,但CpGODN组孕鼠每胎产仔数明显低于CFA组(P<0.05)。病理学分析显示实验小鼠卵巢组织无病理性变化。结论:CpGODN对ZP121-140合成肽诱导的免疫应答和免疫避孕效应略优于CFA,更适合用于避孕疫苗佐剂研究。Objective：To investigate the effect of CpG ODN on the immtme responses and immune-contraception induced by ZP121-140 synthetic peptide. Methods： BALB/c mice were given an injection into the left tibialis anterior muscle of ZP121-140 synthetic peptide with 20 pg CpG ODN or CFA, then the mice were given other injections at 2,4,6 weeks using the same formulation. The mice＇ s blood was collected before each vaccination and after the last vaccination every 2 weeks. The specific IgG and IgA in sera and non-specific cytokines IFN-γ, TNF-α, IL-10 in vaginal mucosa were measured by ELISA. The ovarial pathological changes were tmdertaken using hematoxylin and eosin-stained paraffin sections. Results：The specific lgG in sera and IgA in vaginal mucosa induced by ZP121-140 synthetic peptide combined with CpG ODN were no more than those of ZP121-140 synthetic peptide combined with CFA. There were significant increases in IFN-γ and TNF-α when CpG ODN was mixed with ZP121-140 synthetic peptide and the increase of CpG ODN was more significant than that of CFA. Otherwise there was a significant decrease in IL-10 when CpG ODN was mixed with ZP121-140 synthetic peptide and the decrease of CpG ODN was more significant than thai of CFA. There was no significant difference in the rate of pregnancy between CpG ODN group and CFA group, but the average number of birth mice in CpG ODN group was less than that in CFA group. No pathological changes were found in the ovaries of experimental mice. Conclusion： The adjuvant effect of CpG ODN is more advantageous than that of CFA in contraception vaccine research.

关 键 词：CPG ODN 透明带 合成肽 免疫避孕 

分 类 号：R392.12[医药卫生—免疫学]