中国主栽银耳配对香灰菌的系统发育和遗传多样性  

Phylogeny and Genetic Diversity of Annulohypoxylon spp. Paired with Cultivated Tremella fuciformis Berk

作　　者：温文婷[1] 贾定洪[2] 郭勇[2] 孙群[1] 杨志荣[1] 彭卫红[1,2] 

机构地区：[1]四川大学生命科学学院,成都610064 [2]四川省农业科学院土壤肥料研究所,成都610066

出　　处：《中国农业科学》2010年第3期552-558,共7页Scientia Agricultura Sinica

基　　金：国家自然科学基金资助项目(30972074);四川省财政育种工程资助项目(2006YZCC09-024);四川省农业科学院优秀基金项目(2009YXLWJJ-007)

摘　　要：【目的】了解中国主栽银耳配对香灰菌的分类地位及遗传多样性,为香灰菌资源利用和育种提供分子生物学依据。【方法】采用18S-28S rDNA间隔区(ITS4/ITS5)的PCR扩增和内部简单重复序列多态性(ISSR)分子标记,对中国主栽银耳配对香灰菌的遗传多样性进行分析。【结果】16株供试菌株ITS序列分析表明,供试菌株与Annulohypoxylon stygium关系最为接近,同源性高达99%。从70个ISSR引物中筛选出了11个多态性好的引物,共获得62条ISSR标记。以相关系数0.7为阈值,将16株菌划为5个遗传组,菌株间遗传差异较大,遗传多样性较高。【结论】中国主栽银耳地区的香灰菌遗传资源较为丰富,这为利用香灰菌资源选育新品种提供了可能。[ Objective ] The aim of this paper is to study the classification and genetic diversity of Xianghui and to provide biological information on utilization and breeding of Xianghui. [Method] The phylogeny of 16 strains of Xianghui, which were collected from different provinces and paired with cultivated Tremella, was determined by ITS sequences analysis. Furthermore, the genetic diversity of these strains was analyzed by inter-simple sequence repeat （ISSR）. [Result] The ITS sequences of the 16 strains were analyzed and the phylogenic tree was constructed, and the results revealed that the homology between Xianghui and Annulohypoxylon stygium was 99%. Among the 70 ISSR primers, 11 with high polymorphism were selected and a total of 62 ISSR markers were obtained. At 0.7 similarity distance, cluster analysis revealed the existence of five distinct genetic groups. [Conclusion] The 16 strains of Annulohypoxylon spp. investigated showed low genetic similarity and genetic variations at DNA level significantly.

关 键 词：银耳 伴生菌 遗传多样性 聚类分析 

分 类 号：S567.34[农业科学—中草药栽培] Q943[农业科学—作物学]