PP_(333)诱导黄瓜子叶节差异蛋白表达的分析  

Analysis of Differential Proteins of Cucumber Cotyledonary Nodes Induced by PP_(333)

作　　者：李凤玉[1] 潘德灼[1] 梁海曼[2] 陈伟[3] 

机构地区：[1]福建师范大学生命科学学院,福州350108 [2]浙江大学生命科学学院,杭州310012 [3]福建农林大学生命科学学院,福州350002

出　　处：《中国农业科学》2010年第3期655-660,共6页Scientia Agricultura Sinica

基　　金：福建省自然科学基金项目(B0510007);福建省科技厅项目(K04025)

摘　　要：【目的】了解和寻找PP333处理所导致的差异蛋白表达,为深入研究PP333的作用规律和机理获取有价值的信息。【方法】应用双向凝胶电泳技术研究PP333处理后黄瓜离体子叶节培养物差异蛋白的表达,结合质谱分析和蛋白质数据库检索,确定所检出差异蛋白质的性质和功能。【结果】双向电泳检测到黄瓜离体子叶节培养物蛋白点有1000个左右,质谱(MALDI-TOF-TOF/MS)分析鉴定出5个有信息价值的差异表达蛋白点,分别为14-3-3蛋白(R2)、氨基肽酶(R7)、甲硫氨酸合酶(R11)、60s酸性核糖体蛋白(R20)和PEPC(R21)。这些蛋白质与代谢过程、蛋白质翻译及信号转导等关系密切。【结论】PP333处理导致多种蛋白质表达发生改变,并通过多种蛋白质的相互协调作用调控植物体的生长发育。[ Objective ] The aim of the study was to understand and find differential proteins induced by PP333, which could provide valuable information for further study on its effective law and mechanism. [Method] Two-dimensional electrophoresis （2-DE） was used to study the variation expression of proteins of cucumber cotyledonary nodes induced with PP333. The characteristics and functions of differentially expressed proteins were analyzed with MALDI-TOF-TOF/MS and Protein Data Bank. [Result] Nearly 1 000 proteins were reproducibly detected in 2-DE gel. Five differential proteins were identified, including 14-3-3 protein （R2）, aminopeptidase （R7）, methionine aminopeptidase （R11）, 60s acidic ribosomal protein （R20） and PEPC （R21）. The proteins were closely related to metabolism, translation and signal transduction, which may play an important role in flower bud differentiation of cucumber. [Conclusion] PP333 treatment induced the variation of many proteins which regulated plant growth and development by their mutual coordination.

关 键 词：PP333 黄瓜子叶节 差异蛋白 质谱 

分 类 号：S642.2[农业科学—蔬菜学]