鸡传染性喉气管炎病毒PCR快速检测方法的建立  

Development of polymerase chain reaction(PCR) for rapidly detecting infectious laryngotracheitis virus

作　　者：邵攀峰[1] 陈红英[1] 崔保安[1] 王振亚[1] 宋亚鹏[1] 李森 谢佳喜[1] 

机构地区：[1]河南农业大学牧医工程学院,郑州450002 [2]河南省项城市畜牧局动物卫生监督所,项城466200

出　　处：《安徽农业大学学报》2010年第1期58-62,共5页Journal of Anhui Agricultural University

摘　　要：根据GenBank发表的传染性喉气管炎病毒(ILTV)TK基因序列(EF552578),设计合成了1对特异性引物,通过优化反应条件,成功地从ILTV疫苗株中扩增出329 bp特异性片段,而传染性支气管炎病毒、新城疫病毒及马立克病毒基因组均未扩增为出相应的片段。回收的PCR产物经EcoRⅠ酶切和测序鉴定,证实了该扩增片段的特异性。PCR检测ILTV DNA的最小检测量为30 pg。经临床初步应用表明,该方法的建立使传染性喉气管炎病毒的检测更为快速、简便、经济和实用。A PCR based detection method was developed using a pair of primers designed from a conserved region of the infectious laryngotraeheitis virus （ILTV） TK gene sequence （EF552578） in GenBank. A 329 bp fragment was amplified from ILTV vaccine strain, but not from IBV, NDV and MDV. This amplified fragment is specific for ILTV DNA based on EcoR I digestion pattern and sequencing. The method could be used to detect the template DNA of 30 pg for ILTV. Clinical detection of ILTV by PCR showed that this improved PCR approach could be a fast, simple and specific detection method for ILTV.

关 键 词：鸡传染性喉气管炎病毒 PCR 检测 

分 类 号：S858.31[农业科学—临床兽医学]