The inhibiting effect of 1.4 recombinant P chromosome of wheat-Agropyron cristatum addition line on the Ph gene  

作　　者：YANG GuoHui YANG XinMing WANG RuiHui GAO AiNong LI LiHui LIU WeiHua 

机构地区：[1]National Key Facility for Crop Gene Resources and Genetic bnprovement/lnstitute of Crop Sciences, Chinese Academy of Agricultural Sciences,Beijing 100081, China

出　　处：《Chinese Science Bulletin》2010年第2期153-157,共5页

基　　金：supported by the National High Technology Research and Development Program of China (Grant No. 2006AA10Z174);the National Key Technology Research and Development Program of China (Grant No.2006BAD13B02)

摘　　要：P chromosomes may carry a genetic system that inhibits the Ph gene in wheat. Abnormal chromosome synapsis in wheat-Agropyron cristatum addition line II-21-2 (additional 1·4 recombinant P chromosome) was observed in this study. The results of cytogenetics and Ph1 gene amplification showed that the Ph1 gene was normal and the average number of quadrivalents or hexavalents was determined to be 0.41 and 0.13, respectively, in pollen-mother cells of wheat-Agropyron cristatum addition line II-21-2. The analysis of dual-color GISH/FISH showed that the P chromosomes were not directly involved in the composition of multivalents but could inhibit the effect of the Ph gene, leading to synapsis of wheat homoeologous chromosomes and translocation between wheat homoeologous chromosomes such as 3B-3D chromosomes. The characteristic of P chromosomes’ promoting synapsis of wheat homoeologous chromosomes may have potential application in the genetic improvement of wheat.P chromosomes may carry a genetic system that inhibits the Ph gene in wheat. Abnormal chromosome synapsis in wheat-Agropyron cristatum addition line Ⅱ-21-2 （additional 1-4 recombinant P chromosome） was observed in this study. The results of cytogenetics and Phl gene amplification showed that the Phl gene was normal and the average number of quadrivalents or hexavalents was determined to be 0.41 and 0.13, respectively, in pollen-mother cells of wheat-Agropyron cristatum addition line Ⅱ-21-2. The analysis of dual-color GISH/FISH showed that the P chromosomes were not directly involved in the composition of multivalents but could inhibit the effect of the Ph gene, leading to synapsis of wheat homoeologous chromosomes and translocation between wheat homoeologous chromosomes such as 3B-3D chromosomes. The characteristic of P chromosomes＇ promoting synapsis of wheat homoeologous chromosomes may have potential application in the genetic improvement of wheat.

关 键 词：染色体重组 PH基因 附加系 小麦 抑制作用 冰草 同源染色体 电话 

分 类 号：Q789[生物学—分子生物学] S511.035.1[农业科学—作物学]