Tween-20协同麦草酶水解的研究  

Enzymatic Hydrolysis of Wheat Straw Cooperated with Tween-20

作　　者：罗鹏[1] 刘忠[2] 

机构地区：[1]天津商业大学机械工程学院,天津300134 [2]天津科技大学材料科学与化学工程学院,天津300222

出　　处：《华南农业大学学报》2010年第1期55-59,共5页Journal of South China Agricultural University

基　　金：天津市应用基础与前沿技术研究计划项目(05YFGPGX05700)

摘　　要：采用正交试验设计的方法,对在混合酶(纤维素酶Celluclast1.5 l,β-葡萄糖苷酶Novozym188)与Tween-20协同作用下,经乙醇预处理的麦草酶水解工艺条件进行研究,详细讨论了反应温度、底物浓度、Tween-20用量、纤维素酶用量对还原糖浓度和得率的影响,并对酶水解工艺进行优化.结果表明,最佳工艺条件为反应温度50℃,底物质量浓度100 g.L-1,Tween-20用量0.03 g.g-1,纤维素酶用量15 FPU.g-1.在此条件下,水解72 h时,还原糖质量浓度和得率分别达到46.1 g.L-1和41.5%.Dilute sulfuric acid impregnated and ethanol pretreated wheat straw was used as substrate for enzymatic hydrolysis with Tween-20 additive through orthogonal experiment design. Cellulase mixture （Celluclast 1.5 1 and β-glucosidase Novozym188） were used in combination with Tween-20. The effects of reaction temperature, substrate concentration, Tween-20 loading and enzyme loading were evaluated and the enzymatic saccharification conditions were optimized. The optimum reaction temperature was 50℃, 100 g · L^-1 for substrate concentration,0. 03 g· g^-1 for Tween-20 loading, 15 FPU· g^-1 for enzyme loading. Under the optimum condition, the reducing sugar concentration increased with reaction time ,46. 1 g · L^-1 of the reducing sugar concentration and 41.5 % of the reducing sugar yield were obtained respectively after 72 h.

关 键 词：麦草 Tween-20 酶水解 还原糖 

分 类 号：Q55[生物学—生物化学] TQ91[化学工程]