非小细胞肺癌患者诱导痰中环氧合酶-2和白细胞介素-10表达的变化及其意义  

Expression of cyclooxygenase 2 in sputum of NSCLC patients and its clinical significance

作　　者：黄翠萍[1,2] 杨和平[1,2] 

机构地区：[1]咸宁学院临床医学院 [2]咸宁学院附属中心医院呼吸内科,湖北咸宁437100

出　　处：《临床肺科杂志》2010年第2期153-155,共3页Journal of Clinical Pulmonary Medicine

基　　金：湖北省教育厅课题资助项目(Q200628004)

摘　　要：目的探讨非小细胞肺癌(NSCLC)患者诱导痰中环氧合酶-2(COX-2)和白细胞介素-10(IL-10)的表达变化及其与临床病理参数之间的关系。方法采用逆转录聚合酶联反应(RT-PCR)、放射免疫法和酶联免疫吸附试验(ELISA),分别检测58例NSCLC患者和20例正常健康者痰液中COX-2 mRNA表达、PGE2和IL-10浓度的变化。结果20例正常对照组痰液中仅有2例检测到微弱的COX-2 mRNA表达,阳性表达率为10%;58例肺癌患者痰液中有49例检测到明显的COX-2 mRNA表达,阳性表达率为84.48%,两者比较差异具有显著性(P<0.01)。半定量分析发现肺癌患者痰液中COX-2 mRNA表达的平均吸光度较正常对照组显著升高(P<0.001)。肺癌患者诱导痰上清液中PGE2和IL-10含量分别为(42.69±9.36)μg/L和(32.85±10.84)μg/L,与健康对照者比较,差异分别具有显著性(P<0.01)。直线相关分析发现COX-2 mRNA的表达和PGE2、IL-10浓度之间分别呈正相关(r=0.699,0.587,P<0.05)。肺癌患者痰液中COX-2 mRNA表达与患者性别、年龄、组织分型、TNM分期和分化程度之间无相关性。结论NSCLC患者痰液中COX-2 mRNA、PGE2和IL-10表达增强,对NSCLC诊断具有重要意义。COX-2与NSCLC的发生和发展密切相关。Objective To explore the expression of cyclooxygenase 2 （COX-2） and intedeukin-10 （IL-10） in sputum of non-small cell lung cancer （ NSCLC ） patients and its clinical significance. Metheds By reverse-transcriptional polymerase chain reaction （ RT- PCR） ,RIA and ELISA,the expression of COX-2 mRNA,PGE2 and IL-IO in sputum of 58 cases of NSCLC patients and 20 cases normal health were measured. Results Only two cases in the normal group were measured to express COX-2 mRNA,with a positive percentage of 10%. There were 49 cases in the lung cancer group to express COX-2 mRNA,with a positive percentage of 84.48%. There was a significant difference between the express COX-2 mRNA of the two groups（ P 〈 0. 01 ）. Quantitative analysis found that the average absorbanee of COX-2 mRNA expression in the lung cancer group was significantly higher than that in the normal group（ P 〈0. 001 ）. The concentration of PGE2 and IL-10 in sputum of NSCLC patients were significantly higher than those of normal health group（ P 〈0. 01 ）. There was a good positive correlation between the expression of COX-2 mRNA, PGE2 and IL-10（ r =0. 699,0. 587, P 〈0. 05 ）. The expression of COX-2 mRNA in sputum had no relation to sex, age, histopathologieal type, TNM staging and differentiation degree （ P 〉 0.05 ）. Conclusion The expression of COX-2 mRNA,PGE2 and IL-10 in sputum increased in NSCLC patients,which may play an important role in carcinogenesis and development of lung cancer.

关 键 词：肺癌 环氧合酶 诱导痰 白细胞介素-10 

分 类 号：R734.2[医药卫生—肿瘤]