内源性NO对人乳腺癌细胞化疗敏感性的影响  

Effects of endogenous NO on sensitivity to chemotherapy in human breast cancer cell line

作　　者：涂巍[1] 温健[1] 乔婉晴[1] 赵嫚[1] 于作夫[1] 

机构地区：[1]中国医科大学附属第四医院乳腺外科,沈阳110032

出　　处：《国际外科学杂志》2010年第1期32-35,共4页International Journal of Surgery

摘　　要：目的研究内源性一氧化氮（NO）对人乳腺癌细胞化疗敏感性的影响。方法应用IL—1β处理培养的MCF-7细胞，检测NO的产生情况并用蛋白质印迹法检测诱导型一氧化氮合酶（induceble nitric oxide synthase，iNOS）蛋白的表达。MTT法检测MCF-7细胞在NOS抑制剂NG-甲基-L-精氨酸（NG-monomethyl-L-arginine，L-NMMA）和NO合成原料L-精氨酸（L—arginine，L—Arg）作用下对多柔比星（adriamycin，ADM）和5-氟尿嘧啶（5-fluorouracil，5-Fu）药物的敏感性。结果内源性NO的产量与IL-1β剂量呈正相关。在IL-1β诱导作用下MCF-7细胞大量表达iNOS蛋白，并与L-Arg、L—NMMA存在与否无关。当ADM浓度为0．5μmol／L和1μmol／L时，实验组细胞的生存率明显下降（P〈0．05）。加入L-NMMA能显著提高实验组细胞的生存率（P〈0．05）；加入L—Arg能显著提高MCF-7细胞对化疗药物的敏感性（P〈0．05）。结论在细胞因子IL-1β诱导下MCF-7细胞产生的内源性NO增加，并使MCF-7细胞化学敏感性提高.Objective To evaluate the effects of endogenous NO on the ehemosensitivity of human breast cancer cell. Methods MCF-7 cells were euhured as monolayer, incubated with eytokine IL-1β. The produetion of NO was detected by NO assay. The expression of iNOS protein was measured by Western blotting. Establishing control group and experimental group, the chemosensitivity of MCF-7 cells incubated by L- NMMA and L-Arg to ADM and 5-Fu was studied by MTT assay. Results There was a positive correlation of dose-dependence between NO production and IL-1β concentration. MCF-7 cells expressed plenty of iNOS by induction of IL-1β. There was no significant difference on iNOS whether L-NMMA and L-Arg existed or not, Incubating MCF-7 cells with 0.5 μmol/L or 1 μmol/L ADM, the survival rate of experiment group was remarkablely decreased（P 〈 0.05 ） ; L-NMMA significantly increased survival rate of experiment group（ P 〈 0.05） ; L-Arg decreased survival rate of experiment group（ P 〈 0.05）. Conclusion The induction of IL- 1β in MCF-7 cells can increase the production of endogenous NO, which increases MCF-7 cells＇ sensitivity to chemotherapy.

关 键 词：乳腺癌 一氧化氮 白细胞介素-1Β 化学敏感性 

分 类 号：R737.9[医药卫生—肿瘤]