基于Sol-gel膜和多壁碳纳米管/铂纳米颗粒增效的电流型L-乳酸生物传感器  

Amperometric L-lactate Biosensor Based on Sol-Gel Film and Multi-walled Carbon Nanotubes/Platinum Nanoparticles Enhancement

作　　者：贺晓蕊[1] 于京华[1] 葛慎光[1] 张秀明[1] 林青[1] 朱晗[2] 封烁[3] 袁靓[3] 黄加栋[3] 

机构地区：[1]济南大学化学化工学院,济南250022 [2]济南大学泉城学院,济南250022 [3]济南大学医学与生命科学学院,济南250022

出　　处：《分析化学》2010年第1期57-61,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(Nos.30700535;30972056);国家863计划(No.2007AA10Z430);中韩(NSFC-NRF)联合资助合作与交流项目(No.30911140278)资助

摘　　要：构建了基于多壁碳纳米管(Multi-walled carbon nanotubes,MWCNTs)和铂纳米颗粒(Pt-nano)的电流型L-乳酸生物传感器。将Sol-gel膜覆盖在L-乳酸氧化酶(L-lactate oxidase,LOD)和MWCNTs/Pt-nano修饰的电极表面。实验结果表明:传感器的最佳工作条件为:检测电压0.5V;缓冲液pH6.4;检测温度25℃。此传感器的响应时间为5s,灵敏度是6.36μA/(mmol/L)。连续检测4星期其活性仍保持90%,线性范围为0.2～2.0mmol/L,且抗干扰能力强。在实际血样的检测中,此传感器与传统的分光光度法具有很好的一致性。An electrochemical L-lactate biosensor was fabricated by combining Platinum nanoparticles （Pt-nano） with multi-walled carbon nanotubes（MWCNTs）.L-lactate oxidase（LOD） was immobilized on the surface of the glassy carbon electrode（GCE） modified with MWCNTs and Pt-nano.The surface of resulting LOD/MWCNTs/Pt-nano electrode was covered by a thin layer of sol-gel to avoid the loss of LOD and to improve the anti-interference ability.The cyclic voltammetric results indicated that MWCNTs/Pt-nano catalyst displayed a higher performance than MWCNTs.Under the optimized conditions,i.e.,applied potential of 0.5 V,pH 6.4,25 ℃,the proposed biosensor＇s determination range was 0.2-2.0 mmol/L,response time was within 5 s,and the sensitivity was 6.36 μA/（mmol/L）.It still kept 90% activity after 4 weeks.The fabricated biosensor had practically good selectivity against interferences.The results for whole blood samples analyzed by the present biosensor showed a good agreement with those analyzed by spectrophotometric method.

关 键 词：生物传感器 L-乳酸 溶胶-凝胶 铂纳米颗粒 多壁碳纳米管 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]