双酚A对激素相关肿瘤细胞增殖活性的影响  

Effects of bisphenol A on the proliferation of three kinds of hormone-related tumor cells

作　　者：刘文敏[1] 许强[1] 阴海鹏[2] 李翠玲[2] 孙晓萌[1] 顾洪涛[2] 张玲[2] 

机构地区：[1]山东省医学科学院基础医学研究所卫生检验中心,济南250062 [2]山东省医学科学院基础医学研究所山东医药卫生肿瘤免疫与中药免疫重点实验室

出　　处：《山东医药》2010年第1期9-11,共3页Shandong Medical Journal

基　　金：山东省人口和计划生育委员会科学技术研究项目[(2007)年第(16)号]

摘　　要：目的研究环境雌激素双酚A(BPA)对人卵巢癌3AO细胞、子宫内膜癌HHUA细胞及前列腺癌DU145细胞株增殖活性的影响,探讨BPA的致瘤性。方法将不同浓度的BPA以及17β-雌二醇(17β-E2)作用于3AO、HHUA及DU145细胞,并设溶剂对照,采用MTT法测定药物作用48 h的细胞增殖情况,并以浓度为10-9mol/L的BPA、10-7mol/L的E2作用于三种细胞,检测药物作用0、24、48、72 h的细胞增殖活性。结果与溶剂对照相比,BPA分别在10-5、10-9mol/L的浓度促进3AO、HHUA及DU145细胞增殖(P<0.05);10-9mol/LBPA作用48、72 h对HHUA及DU145细胞均有促增殖效应,与溶剂对照相比,差异有统计学意义(P<0.05)。结论BPA可促进激素相关肿瘤细胞3AO、HHUA及DU145的增殖,且其促进肿瘤细胞的增殖效应与剂量、时间相关。提示BPA的污染可能与激素相关性肿瘤的发生发展有关。Objective To study the effect of bisphenol A （BPA） on the proliferation of 3AO, HHUA and DU145 cells. Methods 3AO cells, HHUA cells and DU145 cells were exposed to BPA and 17β-Estradiol （E2） in different concentrations. The proliferation activities of different cells were detected by MTr assay at 48 h. Then the absorbance values of different cells treated with 10^-9 mol/L BPA or 10^-7 mol/L E2 were obtained at 0 h, 24 h, 48 h and 72 h. Results Compared with solvent control, BPA could significantly stimulate 3AO, HHUA and DU145 ceils to proliferate in the concentration of 10^-5 ,10^-9 mol/L at 48 h （P 〈 0.05）. In addition, 10^-9 mol/L BPA could significantely promote HHUA and DU145 cells to proliferate in a time-dependent manner（ P 〈 0.05）. Conclusions The contamination of BPA might be associated with the occurrence and development of hormone-related tumors.

关 键 词：环境雌激素 双酚A 3AO细胞 HHUA细胞 DU145细胞 细胞增殖 

分 类 号：R730.3[医药卫生—肿瘤]