卵巢上皮性癌淋巴结转移相关差异表达蛋白内质网氨基肽酶1的表达及意义  

Expression and significance of differentially expressed protein endoplasmic reticulum aminopeptidase 1 in ovarian carcinoma with lymph node metastasis

作　　者：刘冰[1] 黎丹戎[1] 倪萍[1] 李力[1] 

机构地区：[1]广西医科大学附属肿瘤医院妇瘤科,南宁530021

出　　处：《中华妇产科杂志》2010年第1期41-44,共4页Chinese Journal of Obstetrics and Gynecology

基　　金：基金项目：高等学校博士学科点专项科研基金（20050598002）;广西自然科学基金（桂科自0728193,桂科能0842009-Z9）

摘　　要：目的 从基因转录及蛋白表达的水平检测内质网氨基肽酶1（ERAP1）在卵巢上皮性癌（卵巢癌）细胞、组织中的表达，探讨其与卵巢癌转移的关系。方法应用实时荧光定量PCR技术、蛋白印迹法检测在卵巢癌淋巴结非定向转移细胞系（SKOV3）与淋巴结定向高转移细胞系（SKOV3-pm2、SKOV3-pm3、SKOV3．pr04）中ERAP1mRNA和蛋白的表达情况；并应用免疫组化方法在人卵巢癌原发灶与相应的淋巴结转移灶、裸鼠卵巢癌移植瘤原发灶与相应的淋巴结转移灶中对ERAP1的蛋白表达进行验证。结果SKOV3、SKOV3-pm／2、SKOV3-pm3、SKOV3-pm4细胞中ERAP1mRNA和蛋白的表达呈下降趋势（分别为0．118±0．012、0．031±0．003、0．028±0．003、0．016±0．005；0．91±0．33、0．09±0．03、0．10±0．04、0．05±0．04），其中，SKOV3细胞中ERAP1mRNA和蛋白的表达显著高于SKOV3-pm2、SKOV3-pm3、SKOV3-pm4细胞，分别比较，差异均有统计学意义（P〈0．05）。ERAP1蛋白在人卵巢癌原发灶（184±14）和相应的淋巴结转移灶、裸鼠卵巢癌移植瘤原发灶（143±22）和相应的淋巴结转移灶（97±12）中的表达均旱下降趋势，分别比较，差异均有统计学意义（P〈0．05）。结论卵巢癌中ERAP1表达的下降或缺失与淋巴结转移有关。Objective To detect the genetic transcription and protein expression level of endoplasmic reticulum aminopeptidase 1 （ERAP1） in human ovarian cancer cells and tissue and study their relationship with directional lymphatic metastasis. Methods Real-time fluorescent quantitative PCR and western blot were used to determine the expressions of ERAPI gene and protein in the human ovarian cancer cell lines between non-directional （SKOV3） and directional highly lymphatic metastasis （SKOV3-pm2, SKOV3-pm3, SKOV3-pm4）. lmmunohistochemistry method was used to further validate the ERAP1 expressions of the transplanted ovarian tumor primarily focus and the lesions of lymph node metastasis from nude mice and the human ovarian cancer primarily focus and the lesions of lymph node metastasis. Results The expression level of ERAP1 gene and protein were down-regulated in SKOV3, SKOV3-pm2, SKOV3-pro3, SKOV3-pm4 cell sublines （0. 118 ± 0. 012, 0. 031 ±0. 003, 0. 028 ± 0. 003,0. 016 ±0. 005 ; 0. 91 ±0. 33, 0. 09 ±0. 03, 0. 10 ±0. 04, 0. 05 ±0. 04; respectively）, and the level of ERAP1 in SKOV3 cell was higher than those in the other three kinds of cell lines （P 〈 0. 05）. The results showed that there were significant declining trend of expression of ERAP1 in the human ovarian cancer cell lines between nondirectional and directional highly lymphatic metastasis; the transplanted ovarian tumor primarily focus and the metastasis lesions of lymph node from nude/nice （ 143 ±22 vs. 97±12, P 〈 0. 05）, the primarily focus （184± 14） and the lesions of lymph node metastasis from human ovarian tumors （P 〈0. 05）. Conclusion The absence or down-regulated expression of ERAP1 is closely related to the metastasis and invasion of lymph node in ovarian carcinoma.

关 键 词：卵巢肿瘤 淋巴转移 氨肽酶类 

分 类 号：R737.31[医药卫生—肿瘤]