重组人生长激素对脑缺血/再灌注损伤细胞凋亡及Nestin表达的影响  

Effects of recombinant human growth hormone on apoptosis and expression of Nestin in rats with cerebral ischemia-reperfusion injury

作　　者：刘海涛[1] 白宏英[1] 曾志磊[1] 郭耀强[1] 方李鸿[1] 

机构地区：[1]郑州大学第二附属医院神经内科,郑州450014

出　　处：《中国实用神经疾病杂志》2010年第1期39-41,共3页Chinese Journal of Practical Nervous Diseases

摘　　要：目的观察重组人生长激素(recombinant human growth hormone,rhGH)对脑缺血/再灌注(ischemia-reperfu-sion,I/R)损伤后神经元细胞凋亡及Nestin表达的影响。方法54只SD雄性大鼠随机分成3组:假手术组、对照组(脑缺血/再灌注+生理盐水组)、治疗组(脑缺血/再灌注+重组人生长激素),每组18只。应用线栓法制作大鼠脑缺血/再灌注损伤模型,大脑中动脉缺血2h,分别在再灌注7d、14d、21d后处死,采用TUNEL法,免疫组织化学法检测顶叶皮质内7d、14d、21d的神经元凋亡和巢蛋白(Nestin)的表达。结果应用重组人生长激素干预后顶叶皮质在7d、14d、21d神经元凋亡数较大鼠对照组明显减少,Nestin的表达显著增加。结论rhGH的神经保护作用可能与抑制缺血神经元细胞凋亡和上调Nestin的表达有关。Objective To investigate the effect of the recombinant human growth hormone （rhGH） on apoptosis and the expression of Nestin in male SD rats with cerebral ischemia-reperfusion injury. Methods The middle cerebral artery ischemiareperfusion injury model was established by middle cerebral artery occlusion （MCAO） with nylon suture. Fifty-four rats were randomly divided into 3 groups： the sham group （n=18）, the control group （n= 18） and the treatment group （n= 18）. We used TUNEL and immunohistochemical method to detect TUNEL-positive apoptotie cells and the expression of Nestin in cortex of parietal lobe on the 7th, 14th and 21st day after isehemia-reperfusion injury. Results TUNEL-positive apoptotic cells were decreased in treatment group on the 7th, 14th and 21st day, and the expression of Nestin was increased obviously in cortex on the 7th, 14th and 21st day. Conclusion rhGH could inhibit neural apoptosis and increase the expression of Nestin involved in the neuroprotection of rhGH.

关 键 词：缺血/再灌注 TUNEL 重组人生长激素 NESTIN 

分 类 号：R-33[医药卫生]