高效毛细管电泳法测定白胡椒中胡椒碱的含量  

Determination of Piperine in Fructus piperis by High Performance Capillary Electrophoresis

作　　者：李和光[1] 周洪雷[1] 蒋海强[1] 杜昊忱[1] 李飞[1] 

机构地区：[1]山东中医药大学,山东济南250355

出　　处：《辽宁中医杂志》2010年第1期134-135,共2页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)资助项目(2007CB512601)

摘　　要：目的:测定中药白胡椒中胡椒碱的含量。方法:采用高效毛细管电泳法,电泳条件为石英毛细管柱(50μm×90cm,实际长度81.5cm),运行缓冲液为0.02mol/LNa2HPO4:0.02mol/LNaH2PO4=95:5(16℃时pH=8.6),分离电压为28kV,压力进样30mbar×8s,温度为25℃,检测波长为343nm。结果:胡椒碱在0.05～0.30mg/mL范围内呈良好的线性关系(r=0.9995),平均回收率分别为99.82%,99.95%,100.20%;RSD分别为0.30%,0.69%,0.29%(n=3)。结论:高效毛细管电泳法可用于测定白胡椒中胡椒碱的含量。Objective：To determine the content of Piperine in Fructus piperis.Methods：Apply the methods of HPCE,and the best condition was using the quartz capillary tube（50μm×90cm,practical length is 81.5cm）,0.02mol/L Na_2HPO_4：0.02mol/L NaH_2PO_4=95：5（pH=8.6 at 16℃）buffer,pressure injection 30mbar×8s,at a constant voltage of 28kV and temperature at 25℃.It was detected on 343nm.Results：Piperine had a good linearity within 0.05~0.30mg/mL（r=0.9995）,and the average recovery were 99.82%,99.95%,100.20% with RSD 0.30%,0.69%,0.29%（n=3）.Conclusion：This method is feasible for determination of Piperine in Fructus piperis.

关 键 词：白胡椒 胡椒碱 高效毛细管电泳 

分 类 号：R284.1[医药卫生—中药学]