绵羊BMPR-IB基因在分子标记育种中的应用效果  

Research on Application Effect in Breeding of Molecular Marker in Sheep BMPR-IB Gene

作　　者：方翟[1] 吴建平[1] 徐建峰[1] 罗艳茹[2] 张利平[1] 王磊[1] 杨联[1] 雷赵民[1] 

机构地区：[1]甘肃农业大学动物科学技术学院,甘肃兰州730070 [2]中国农业大学动物科学技术学院,北京100194

出　　处：《新疆农业科学》2010年第1期189-194,共6页Xinjiang Agricultural Sciences

基　　金：甘肃省农业生物技术研究与应用开发项目(GNSW-2005-11)

摘　　要：【目的】分析小尾寒羊、德国美利奴、德国美利奴(♂)和小尾寒羊(♀)杂交一代(简称DF1)共204只个体的单核苷酸多态性及其与产羔数间的关系。【方法】以绵羊BMPR-IB基因作为候选基因,采用PCR-sscp检测方法。【结果】B等位基因在小尾寒羊、德国美利奴、德寒杂交一代中的频率分别为0.660、0.532和0.600,基因频率及基因型频率在3个群体间的分布差异不显著(P>0.05)。BB、B+、++型的产羔数分别为2.86、2.27和2.08,BB型显著高于B+和++型(P>0.05),B+型显著高于++型(P>0.05)。【结论】在以小尾寒羊为母本的杂交育种过程中,B等位基因是有效的辅助标记基因。[ Objective and Method] The polymorphism of bone morphogenetic protein receptor type IB gene （ BMPR - IB gene ） of 204 sheep of three breeds or strains , which included Small Tail Han sheep, F1 generation from German Merino（ ♂ ） x Small Tail Han sheep（ ♀ ） cross generation （DF1）, German Merino ,was analyzed by PCR - sscp. The relationship between genotype and litter size was also identified. [Result and Conclusion]The results showed that the frequency of B allele in Small - Tail Han sheep, German Merino and DF1 was O. 660,0. 532 and 0. 600, there was no significant difference of distribution of gene frequency and genotype frequency in 3 groups. The litter size of BB, B ＋ and ＋ ＋ was 2.86,2.27 and 2.08, BB were both significantly higher than B ＋ and ＋ ＋ （ P 〈 0.05）, and B ＋ were significantly higher than ＋ ＋ type.

关 键 词：绵羊 BMPR—IB 产羔数 相关性 

分 类 号：S826[农业科学—畜牧学] S188[农业科学—畜牧兽医]