酒糟中蛋白质的提取工艺  

Extraction Conditions of Grains Protein

作　　者：姜福佳[1] 王玉萍[1] 周畅[1] 张肖建[1] 周杰[1] 孟庆繁[1] 

机构地区：[1]吉林大学生命科学学院,长春130012

出　　处：《吉林大学学报（理学版）》2010年第1期152-156,共5页Journal of Jilin University:Science Edition

基　　金：吉林省科技发展计划项目基金(批准号:20096031);2008年生命科学与技术大学生创新实践基地创新实验基金(批准号:JDCX2008017)

摘　　要：采用响应面法优化酒糟中蛋白质的提取条件.在单因素实验的基础上,选取醇碱比、固液比、提取时间为影响因子,应用Box-Behnken中心组合进行3因素3水平的实验设计,以蛋白质提取率作为响应值,进行响应面分析.结果表明,酒糟中蛋白质最佳提取条件为:提取时间87.2min,固液比(g/mL)为1:42.3,醇碱比为1:2.7,提取率预测值19.57%,验证值19.40%,与预测值的相对误差0.88%.The response surface analysis methodology （RSM） was used for optimizing extraction conditions in the extraction process of grains protein.Based on the single-factor experiments,three independent variables,alcohol to alkali ratio,solid to liquid ratio and extracting time were selected as casual factors for the extraction.The experiments were carried out according to Box-Behnken central composite experiment design.Response surface analysis method was applied to determining the effect of central composite experiment design.Response surface analysis method was applied to determining the effects of casual factors on the yield of grains protein.The results show that the optimum extraction conditions for the extraction of grains protein were alcohol to alkali ratio was 1：2.7,solid to liquid ratio（g/mL） 1：42.3,and extracting time was 87.2 min.The predictive best extraction rate was 19.57%,the actual extraction rate 19.40% and the relative error 0.88%.

关 键 词：响应面分析法(RSM) 酒糟 蛋白质 提取工艺 

分 类 号：Q503[生物学—生物化学]