快速检测小麦和玉米中的脱氧雪腐镰刀菌烯醇  

Rapid Detection of Deoxynivalenol in Wheat and Maize

作　　者：黄志兵[1] 许杨[1] 张泓[1] 何庆华[1] 李燕萍[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,南昌大学中德联合研究院,江西南昌330047

出　　处：《江西师范大学学报（自然科学版）》2009年第6期712-715,共4页Journal of Jiangxi Normal University(Natural Science Edition)

基　　金：国家“863”计划项目(2007AA10Z427);江西省科技厅重点攻关项目(S00049)资助

摘　　要：采用DON快速检测试纸条检测小麦和玉米样品,并对检测过程进行了优化,最后采用ELISA试剂盒进行比较和分析.结果发现14号和15号小麦样品为阳性,其余样品均为阴性,两种方法的检测结果一致;样品的颗粒大小、水质、搅拌时间和静置时间等对试纸条的检测结果无明显影响,显示该试纸条适合现场快速筛查.Colloidal gold immunochromatographic strip（ICS） test for rapid detection of deoxynivalenol（DON） in wheat and maize samples was carried.Analysis of DON in wheat and maize samples revealed that data obtained from ICS test were in a good agreement with those obtained from ELISA.The test results have no obvious effect by these factors such as particle size,types of water,stirring time,standing time,etc.The results demonstrate that the ICS test can be used as qualitative tool for screening technique of DON on-site.

关 键 词：脱氧雪腐镰刀菌烯醇 胶体金免疫层析 小麦 玉米 

分 类 号：O655[理学—分析化学]