E-selectin和ICAM-1在肝癌细胞与血管内皮细胞黏附作用中的实验研究  

The effects of E-selectin and ICAM-1 on the adhesion of hepatocellular carcinoma cells and vascular endothelial cells

作　　者：李强[1] 陈德[1] 钱世鹍[1] 李悦[1] 杨学伟[1] 胡海[1] 林航[1] 邓武坚[1] 肖骏琦[1] 陈松伟[1] 

机构地区：[1]广州医学院第二附属医院肝胆外科,广州510260

出　　处：《岭南现代临床外科》2009年第5期327-330,共4页Lingnan Modern Clinics in Surgery

摘　　要：目的探讨E-selectin(内皮细胞选择素)和ICAM-1(细胞间粘附分子-1)在肝癌细胞与血管内皮细胞黏附中的作用:筛选有临床应用前景、能预防肝癌复发与转移的抗黏附药物。方法实时荧光定量PCR检测E-selectin和ICAM-1mRNA在肝静脉内皮细胞ED25和脐静脉内皮TNFα刺激后细胞ECV304上的表达;应用体外粘附实验检测肝癌细胞HepG2与活化的ED25和ECV304的粘附,并检测不同药物对抗粘附作用的影响。结果 E-selectin和ICAM-1可高表达于活化后的ED25和ECV304;且能介导HepG2与ED25和ECV304的粘附;地塞米松、丹参酮ⅡA有较强的抑制HepG2与ED25粘附的作用。结论 E-selectin和ICAM-1可促进肝癌细胞与内皮细胞的粘附;地塞米松、丹参酮ⅡA可起到抗肝癌细胞与血管内皮细胞粘附的作用。Objective To study the role of E-selectin and ICAM-1 on the adhesion of hepatocellular carcinoma cells and vascular endothelial cells,and to select related possible antiadhesion drugs to inhibit recurrence and metastasis of hepatocelluar carcinoma. Methods Expression of E-selectin and ICAM-1 in hepatic vein endothelial cells （ED25） and umbilical veinendothehal cells （ECV304）wcre detected by real-time quantitative PCR. Adhesion between the HepG2 cells and ED25 or ECV304 was examined by sohd phase adhesion assay in vitro. A number of drugs were tested for the affects on the tumor cell-endothelial adhesion. Results E-selectin and ICAM-1 were highly expressed on the surface of actived ED25 and ECV304, and could mediate the adhesion between HepG2 and ED25 or ECV304. Dexamethasone and tanshinone Ⅱ A were able to block the adhesion between HepG2 and ED25 at low concentration. Conclusion E-selectin and ICAM-1 are important moleculars on the adhesion of hepatoeellular carcinoma cells and endothelial cells. Dexamethasone and tanshinone Ⅱ A can be hopefully used as anti-adhesion drugs to inhibit the adhesion of hepatocellular carcinoma cells and vascular endothelial ceils.

关 键 词：肝细胞癌 复发和转移 细胞粘附分子 

分 类 号：R735.7[医药卫生—肿瘤]