谷氨酸促脑胶质瘤细胞侵袭的机制研究  

Study on the mechanism of invasion evoked by glutamine in cerebral glioma cells

作　　者：袁忠民[1] 徐利民[1] 王建刚[1] 李明昌[1] 陆永建[1] 何伟文[1] 

机构地区：[1]广州医学院第二附属医院,广州医学院神经科学研究所(广东省重点实验室,神经遗传与离子通道病省部共建教育部重点实验室),广东广州510260

出　　处：《岭南现代临床外科》2009年第6期456-458,共3页Lingnan Modern Clinics in Surgery

基　　金：广州市医药卫生科技一般引导项目（2009-YB-154）

摘　　要：目的探讨谷氨酸促胶质瘤细胞侵袭的机制,检测MMP-2,MMP-9和uPAR的表达和激活。方法体外培养胶质瘤细胞株U251-MG,分别用50μM和100μM谷氨酸作用24后,Matrigel方法检测细胞侵袭;用含有100μM谷氨酸或同时含有谷氨酸受体阻断剂2μM MK-801的培养基作用U251-MG 24小时,收集细胞用相对定量PCR检测MMP-2及MMP-9 mRNA表达水平,收集上清后进行凝胶酶谱分析(Zymography Assay)检测MMP-2、MMP-9的活性变化;检测谷氨酸刺激后uPAR的mRNA表达水平。结果谷氨酸浓度依赖的促进胶质瘤细胞株U251-MG侵袭;谷氨酸刺激没有改变U251-MG细胞中MMP-2和MMP-9表达水平,但使MMP-2活性增强,MMP-9活性没有变化:谷氨酸上调uPAR的表达。结论谷氨酸可能通过上调uPAR促进胶质瘤细胞发生MMP-2活性依赖的侵袭。Objective To investigate the mechanism of invasion evoked by glutamine in glioma and detect the expression and activation of MMP-2,MMP-9 and uPAR. Methods Glioma cell strain U251-MG was cultured in vitro.50 M and 100 M glutamine were respectively used to 24 hours. The cellular invasion was dected by Matrigel method.Contained glutamine receptor blocker 2 μM MK- 801 culture medium was used to U251-MG for 24 hours. After collecting cells,the expression levels of MMP-2 or MMP-9 mRNA were detected by relative quantitative PCR.Afler collecting supernate,the Zymography assay was performed and the active changes of MMP-2,MMP-9 were detected. Expression levels of mRNA of uPAR were detected after stimulating glutamine. Results Glutamine concentrationdependence promoted invasion of glioma cell strain U251-MG. Stimulation of glutamine didn＇t change expression levels of MMP-2 and MMP-9 in U251-MG cells. But the MMP-2 activity was enheanced and there was no change in the MMP-9 activity. Expression of uPAR was up-regulated by glutamine. Conclusion Glutamine may promote MMP-2 dependent invasion of glioma cells through up-regulatlon of uPAR.

关 键 词：谷氨酸 胶质瘤 肿瘤侵袭 MMP-2 UPAR 

分 类 号：R739.41[医药卫生—肿瘤]