突变型Nbs1基因转染对头颈癌放疗敏感性影响的实验研究  

The experimental study on enhancive radio-sensitivity of head & neck cancer cells after transfected with mutant Nbs1

作　　者：王代友[1] 周诺[1] 张斌[1] 陆新萍[1] 方首镕[1] 蒋磊[1] 

机构地区：[1]广西医科大学附属口腔医院口腔颌面外科,南宁530021

出　　处：《中华口腔医学研究杂志（电子版）》2009年第6期8-10,共3页Chinese Journal of Stomatological Research(Electronic Edition)

基　　金：广西自然科学基金资助(桂科自0640095)

摘　　要：目的探讨突变型Nbs1基因转染对头颈癌细胞放射敏感性的影响。方法用突变型Nbs1腺病毒载体转染头颈癌细胞株JHU006,同时给予2GyCo60γ射线照射,24h后用MTT法测定细胞生长曲线,以及中性彗星法检测癌细胞DNA双链断裂程度。结果JHU006细胞株转染突变型Nbs1基因并配合放射治疗后,癌细胞处于生长停滞状态;中性彗星实验显示,该组癌细胞平均尾力矩值明显增大。结论突变型Nbs1基因转染可以抑制头颈部DNA双链断裂癌细胞的损伤修复功能,因而显著增加了癌细胞放疗敏感性。Objective To determine the radio-sensitivity of head ＆ neck cancer cells after transfected with mutant Nbs1 gene. Methods JHU006 cells were transfected with mutant Nbs1 and then irradiated with 2 Gy of Co60^ γ-ray for 24 hours. The MTT method was applied to determine the growth curve of head ＆ neck cancer cells JHU006 and Neutral comet assay was used for observing the cells＇ DNA Double strain breaks. Results After the treatment, JHU006 cells growth was almost at a standstill and the values of the mean rail moment remarkably increased. Conclusions Transfected with mutant Nbs1 gene, Head ＆ neck cancer cells＇ radio-sensitivity significantly increased attribute to the inhibition of repair function for cancer cells DNA double strain breaks.

关 键 词：MRN复合体 放射敏感性 头颈癌 

分 类 号：R739.91[医药卫生—肿瘤]