角膜移植排斥反应诊断方法的研究  

Study on diagnosis methods of keratoplasty rejecting

作　　者：袁毅[1,2] 赵世洪[1,2] 崔立飞 张瑞雪 

机构地区：[1]白求恩医科大学三院眼科 [2]沈阳市第四人民医院眼科

出　　处：《中国实验诊断学》1998年第4期201-203,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：探讨角膜移植排斥反应早期诊断方法。方法应用电子顺磁共振（ＥＰＲ）技术和荧光光谱法检测泪液自由基和水溶性脂质过氧化物，对排斥反应发生前及发生时的变化情况进行分析。结果在排斥反应临床症状出现之前，泪液中自由基和水溶性脂质过氧化物已有持续增高，直到临床症状出现。其数值与正常对照组相比有显著性差异（Ｐ＜０．０１）。当临床诊断排斥反应时，两指标增高更明显（Ｐ＜０．０００５），治愈后则恢复正常水平。结论角膜移植术后监测泪液中自由基和水溶性脂质过氧化物的变化，是临床早期诊断排斥反应的可行方法。bjective To investigate early diagnosis methods of keratoplasty rejection.Methods By the use of technology of Electron Paramagnetic Resonamce(EPR)and fluoresent spectroscopy,free radical and water solubility lipid peroxide(WSLP) in tears of patients with keratolasty and rejection were determined.Results The results proved that free radical and WSLP had a continued elevation(P<001) before the clinical symptom appeared.When the rejection occurred both targets increased obviously,as compared with those of the normal control (P<0.0005).Conclusion These results,suggest that determination of the changes of free radical and WSLP in tears after keratoplasty is an advisable method of early clinical diagnosis of rejcetion.

关 键 词：角膜移植 排斥反应 自由基 水溶性 SOD 早期诊断 

分 类 号：R779.65[医药卫生—眼科] R392.4[医药卫生—临床医学]