GSTP1和RASSF1A基因多态性和环境因素与前列腺癌的相关性分析  

Relationship of GSTP1,RASSF1A polymorphisms and environmental agent with susceptibility to prostate cancer:a case-control study

作　　者：徐兴兴[1] 常文军[1] 侯建国[2] 徐丹枫[3] 崔心刚[3] 翟羽佳[1,4] 王国萍[1] 张宏伟[1] 曹广文[1] 

机构地区：[1]第二军医大学基础部流行病学教研室,上海200433 [2]第二军医大学长海医院泌尿外科,上海200433 [3]第二军医大学长征医院泌尿外科,上海200003 [4]海军航空工程学院青岛分院,青岛266041

出　　处：《第二军医大学学报》2010年第1期12-17,共6页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(30671793)~~

摘　　要：目的探讨GSTP1和RASSF1A基因多态性及环境因素和前列腺癌易感性之间的关系。方法采用TaqMan/MGB探针基因分型方法对103例前列腺癌患者和103例正常对照的中国汉族人群基因组DNA进行GSTP1和RASSF1A基因多态性检测,并结合环境因素应用多因素Logistic回归分析吸烟、饮酒、饮茶、猪肉和牛肉每周实际摄入量及各位点基因型与前列腺癌易感性之间的关系。结果GSTP1基因型AA、AG、GG在前列腺癌和对照人群中的比例分别为66.02%、22.33%、11.65%和67.96%、29.13%、2.91%,有统计学差异(χ2=6.35,P=0.04);RASSF1A基因型CC、CA、AA在前列腺癌和对照人群中的比例分别为88.34%,5.83%,5.83%和85.44%,12.62%,1.94%,无统计学差异(χ2=4.63,P=0.10)。多因素Logistic回归分析显示,饮茶者患前列腺癌的危险性是不饮茶者的0.40倍(95%CI,0.19～0.82),吸烟者患前列腺癌的危险性是不吸烟者的3.02倍(95%CI,1.44～6.32)。结论GSTP1和RASSF1A的基因多态性和中国汉族人群前列腺癌的发生无相关性;吸烟是前列腺癌的危险因素,饮茶是保护因素。Objective To investigate relationship of GSTP1,RASSF1A polymorphisms and environmental agent with susceptibility to prostate cancer （ Pca）. Methods The GSTP1 and RASSF1A genotypes were determined by TaqMan/MGB Probe Technology in 103 patients with Pca and 103 normal controls. Multivariate logistic regression model was used to assess the association of smoking,alcohol drinking,tea drinking,weekly pork and beef consumption,and the genetic polymorphisms with the susceptibility to Pca,while taking into consideration of the environmental agent. Results The frequencies of the GSTP1 AA,AG and GG genotypes were 66. 02% ,22. 33% ,and 11. 65% in patients with Pca and 67. 96% ,29. 13% and 2. 91% in controls,respectively,with significant difference found between the two groups （ χ2 = 6. 35,P = 0. 04）. The frequencies of RASSF1A CC,CA and AA genotypes were 88. 34% ,5. 83% ,and 5. 83% in patients with Pca,and 85. 44% ,12. 62% ,and 1. 94% in the controls,respectively,with no significant difference found between the two groups （ χ2 = 4. 63,P = 0. 10）. Multivariate analysis showed a decreased risk in those who had a tea drinking history （ OR = 0. 40,95% CI,0. 19 -0. 82） and an increased risk in those who had a smoking history （ OR = 3. 02,95% CI,1. 44 -6. 32）. Conclusion Our results indicate that GSTP1,RASSF1A polymorphisms are not associated with Pca susceptibility in Chinese Han nationality. Smoking is the risk factor of Pca,and tea drinking is a protective factor against Pca.

关 键 词：前列腺肿瘤 病例对照研究 GSTP1 RASSF1A 遗传多态现象 

分 类 号：R737.25[医药卫生—肿瘤]